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YY1 (NM_003403) Human Over-expression Lysate

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SKU
LC401166
Lyophilized YY1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207198
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003403, NP_003394
Synonyms DELTA; GADEVS; INO80S; NF-E1; UCRBP; YIN-YANG-1
Predicted MW 44.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003394
Locus ID 7528
Cytogenetics 14q32.2
Protein Families WB
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Other "YY1" proteins (3)
SKU Description Size Price
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TP762172 Purified recombinant protein of Human YY1 transcription factor (YY1), Gly157-End, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1
Jin, Wu, Wang et al
Stem Cell Res (2016) 17 (2), 277-284 DOI: 10.1016/j.scr.2016.07.011
Cited by 33
AI Summary: OriGene’s YY1 overexpression plasmid (pCMV6-Entry-YY1) was used in this study to investigate the role of YY1 in smooth muscle cell differentiation from stem cells.
Snippet: from BAIAO. Control (pCMV6-Entry) or a YY1 overexpression plasmid (pCMV6-Entry-YY1, Origene) were transfected into differentiating ES cells using Lipofectamine 3000 according to the manufacturer's instructions. MiRNA ... Cite SaveSaving
miR-34a inhibits the migration and invasion of esophageal squamous cell carcinoma by targeting Yin Yang-1
Nie, Ge, Geng et al
Oncol. Rep. (2015)
Cited by 60
AI Summary: OriGene contributed to this study by providing the YY1 overexpression plasmid, which was used to investigate the role of YY1 in esophageal squamous cell carcinoma cell migration and invasion.
Snippet: The protein expression was evaluated using chemiluminescence and exposure to Kodak film (Kodak, Rochester, NY, USA). YY1 overexpression plasmids and shRNA. YY1 overexpres- sion was obtained from OriGene Technologies Inc. (Rockville, MD, USA). ... Cite SaveSaving
Effect of a Single-Nucleotide Polymorphism in the Cholecystokinin Type A Receptor Gene on Growth Traits in the Hinai-dori Chicken Breed
Rikimaru, Takeda, Uemoto et al
The Journal of Poultry Science (2013) 50 (3), 206
Snippet: The biotiny- lated double stranded probes (20fmol) were incubated with 1μg of YY1 overexpression lysate (LY401166;OriGene, Rockville, MD, USA) using an EMSA kit from Signosis (Sunnyvale, CA, USA) according to the manufacturer's in- structions ... Life Sciences, 56: 601-610
Showing 1-3 of 3 papers.
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