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GALK2 (NM_002044) Human Over-expression Lysate

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SKU
LY400749
Transient overexpression lysate of galactokinase 2 (GALK2), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200475
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002044, NP_002035
Synonyms GK2
Predicted MW 50.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002035
Locus ID 2585
Cytogenetics 15q21.1-q21.2
Protein Families WB
Protein Pathways Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
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Other "GALK2" proteins (8)
SKU Description Size Price
PH300475 GALK2 MS Standard C13 and N15-labeled recombinant protein (NP_002035) 10 ug
$3,360.00
PH316527 GALK2 MS Standard C13 and N15-labeled recombinant protein (NP_001001556) 10 ug
$3,360.00
LC400749 Lyophilized GALK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424379 Lyophilized GALK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY424379 Transient overexpression lysate of galactokinase 2 (GALK2), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP300475 Recombinant protein of human galactokinase 2 (GALK2), transcript variant 1, 20 µg 20 ug
$737.00
TP316527 Recombinant protein of human galactokinase 2 (GALK2), transcript variant 2, 20 µg 20 ug
$737.00
TP721055 Purified recombinant protein of Human galactokinase 2 (GALK2), transcript variant 1 10 ug
$340.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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