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DUSP4 (NM_001394) Human Over-expression Lysate

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SKU
LY400543
Transient overexpression lysate of dual specificity phosphatase 4 (DUSP4), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200640
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001394, NP_001385
Synonyms HVH2; MKP-2; MKP2; TYP
Predicted MW 43 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001385
Locus ID 1846
Cytogenetics 8p12
Protein Families WB
Protein Pathways MAPK signaling pathway
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Other "DUSP4" proteins (7)
SKU Description Size Price
PH300640 DUSP4 MS Standard C13 and N15-labeled recombinant protein (NP_001385) 10 ug
$3,360.00
PH315252 DUSP4 MS Standard C13 and N15-labeled recombinant protein (NP_476499) 10 ug
$3,360.00
LC400543 Lyophilized DUSP4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC403296 Lyophilized DUSP4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403296 Transient overexpression lysate of dual specificity phosphatase 4 (DUSP4), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP300640 Recombinant protein of human dual specificity phosphatase 4 (DUSP4), transcript variant 1, 20 µg 20 ug
$737.00
TP315252 Recombinant protein of human dual specificity phosphatase 4 (DUSP4), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

DUSP4 deficiency caused by promoter hypermethylation drives JNK signaling and tumor cell survival in diffuse large B cell lymphoma
Schmid, Robinson, Scheifinger et al
J. Exp. Med. (2015) 212 (5), 775-792 DOI: 10.1084/jem.20141957
Cited by 71
AI Summary: OriGene contributed to this study by providing overexpression constructs in the pCMV6 vector and the wild-type DUSP4 plasmid (pTCN-BC002671), which were used to assess the functional role of DUSP4 in diffuse large B cell lymphoma.
Snippet: … The JNK inhibitors SP600125 and JNK-IN-8 and the BTK inhibitor Ibrutinib were obtained from Selleckchem. The JNK inhibitor BI-87G3 as well as inhibitors of p38 (SB203580) and ERK1/2 (FR180204) were purchased from Sigma-Aldrich. …A phosphatase-inactive mutant of DUSP4, in which cysteine 280 in the wild-type DUSP4 sequence (pTCN-BC002671, Origene) is replaced by a serine, was generated using GENEART site-directed mutagenesis (Invitrogen) as described previously (Robinson et al., 2001)
Showing 1-1 of 1 papers.
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