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DUSP4 (NM_001394) Human Over-expression Lysate

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SKU
LC400543
Lyophilized DUSP4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200640
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001394, NP_001385
Synonyms HVH2; MKP-2; MKP2; TYP
Predicted MW 43 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001385
Locus ID 1846
Cytogenetics 8p12
Protein Families WB
Protein Pathways MAPK signaling pathway
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Other "DUSP4" proteins (7)
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file-alt Citations

DUSP4 deficiency caused by promoter hypermethylation drives JNK signaling and tumor cell survival in diffuse large B cell lymphoma
Schmid, Robinson, Scheifinger et al
J. Exp. Med. (2015) 212 (5), 775-792 DOI: 10.1084/jem.20141957
Cited by 71
AI Summary: OriGene contributed to this study by providing overexpression constructs in the pCMV6 vector and the wild-type DUSP4 plasmid (pTCN-BC002671), which were used to assess the functional role of DUSP4 in diffuse large B cell lymphoma.
Snippet: … The JNK inhibitors SP600125 and JNK-IN-8 and the BTK inhibitor Ibrutinib were obtained from Selleckchem. The JNK inhibitor BI-87G3 as well as inhibitors of p38 (SB203580) and ERK1/2 (FR180204) were purchased from Sigma-Aldrich. …A phosphatase-inactive mutant of DUSP4, in which cysteine 280 in the wild-type DUSP4 sequence (pTCN-BC002671, Origene) is replaced by a serine, was generated using GENEART site-directed mutagenesis (Invitrogen) as described previously (Robinson et al., 2001)
Showing 1-1 of 1 papers.
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