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p38 (MAPK14) (NM_001315) Human Over-expression Lysate

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SKU
LY400523
Transient overexpression lysate of mitogen-activated protein kinase 14 (MAPK14), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217425
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001315, NP_001306
Synonyms CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38ALPHA; PRKM14; PRKM15; RK; SAPK2A
Predicted MW 41.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001306
Locus ID 1432
Cytogenetics 6p21.31
Protein Families WB
Protein Pathways Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway
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Other "p38" proteins (11)
SKU Description Size Price
PH306605 MAPK14 MS Standard C13 and N15-labeled recombinant protein (NP_620581) 10 ug
$3,360.00
PH317425 MAPK14 MS Standard C13 and N15-labeled recombinant protein (NP_001306) 10 ug
$3,360.00
PH322774 MAPK14 MS Standard C13 and N15-labeled recombinant protein (NP_620583) 10 ug
$3,360.00
LC400523 Lyophilized MAPK14 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC408439 Lyophilized MAPK14 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC408440 Lyophilized MAPK14 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY408439 Transient overexpression lysate of mitogen-activated protein kinase 14 (MAPK14), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY408440 Transient overexpression lysate of mitogen-activated protein kinase 14 (MAPK14), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP306605 Recombinant protein of human mitogen-activated protein kinase 14 (MAPK14), transcript variant 2, 20 µg 20 ug
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TP317425 Recombinant protein of human mitogen-activated protein kinase 14 (MAPK14), transcript variant 1, 20 µg 20 ug
$737.00
TP322774 Recombinant protein of human mitogen-activated protein kinase 14 (MAPK14), transcript variant 4, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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