IL1 beta (IL1B) (NM_000576) Human Over-expression Lysate
$436.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC202079 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_000576, NP_000567 |
| Synonyms | IL-1; IL1-BETA; IL1beta; IL1F2 |
| Predicted MW | 30.7 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_000567 |
| Locus ID | 3553 |
| Cytogenetics | 2q14.1 |
| Protein Families | WB |
| Protein Pathways | Alzheimer's disease, Apoptosis, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway, Type I diabetes mellitus |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
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|---|---|---|---|---|
| PH302079 | IL1B MS Standard C13 and N15-labeled recombinant protein (NP_000567) | 10 ug |
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|
|
| LC400196 | Lyophilized IL1B HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
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|
| TP302079 | Recombinant protein of human interleukin 1, beta (IL1B), 20 µg | 20 ug |
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| TP721027 | Purified recombinant protein of Human interleukin 1, beta (IL1B) | 10 ug |
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| TP721175 | Purified recombinant protein of Human interleukin 1, beta (IL1B) | 10 ug |
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| TP723772 | Purified recombinant protein of Human interleukin 1, beta (IL1B) | 10 ug |
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|
| TP750014 | Recombinant protein of human IL 1 beta (IL1B) produced in E. coli. | 100 ug |
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|
Citations
Prolonged hyperinsulinemia increases the production of inflammatory cytokines in equine digital lamellae but not in striated muscle
Jayathilake, de Laat, Furr et al
Vet J (2023) 303, 106053 DOI: 10.1016/j.tvjl.2023.106053
Jayathilake, de Laat, Furr et al
Vet J (2023) 303, 106053 DOI: 10.1016/j.tvjl.2023.106053
Cited by 2
AI Summary: OriGene's Transient Overexpression Lysate of IL-1β (code LY400196) was used as a positive control for protein expression analysis in this study investigating inflammatory cytokine production in equine tissues during prolonged hyperinsulinemia.
Snippet: Hyperinsulinemia is the key feature of equine metabolic syndrome (EMS) which leads to debilitating sequelae. Hyperinsulinemia-associated laminitis (HAL) is one of the Save;, Transient overexpression lysate of IL-1β (Origene, code LY400196);for IL-6, mouse lung;for TNFα, mouse liver;and for NF-κ B, NF-κB Control cell extracts (Cell signaling, code 9243)
Equine insulin dysregulation causes tissue specific alterations of proinflammatory cytokines and acute phase proteins in a NF-kB independent manner
Jayathilake, Furr, Risco et al
Vet Immunol Immunopathol (2022) 253, 110500 DOI: 10.1016/j.vetimm.2022.110500
Jayathilake, Furr, Risco et al
Vet Immunol Immunopathol (2022) 253, 110500 DOI: 10.1016/j.vetimm.2022.110500
Cited by 6
AI Summary: OriGene's IL1β transient overexpression lysate (product no: LY400196) was used as a positive control for Western blotting to assess IL-1β protein expression in tissue samples in this study.
Snippet: Transient overexpression lysate of IL1 β (ORIGENE, product no: LY400196), IL-6;mouse lung, TNFα;mouse liver, NF-κ B;NF-κB Control Cell Extracts (Cell signaling, product no: 9243). Equal protein loading was confirmed using a housekeeping protein (β actin in adipose tissue and
Showing 1-2 of 2 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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