IL1 beta (IL1B) (NM_000576) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC202079 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_000576, NP_000567 |
| Synonyms | IL-1; IL1-BETA; IL1beta; IL1F2 |
| Predicted MW | 30.7 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_000567 |
| Locus ID | 3553 |
| Cytogenetics | 2q14.1 |
| Protein Families | WB |
| Protein Pathways | Alzheimer's disease, Apoptosis, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway, Type I diabetes mellitus |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
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|---|---|---|---|---|
| PH302079 | IL1B MS Standard C13 and N15-labeled recombinant protein (NP_000567) | 10 ug |
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|
|
| LY400196 | Transient overexpression lysate of interleukin 1, beta (IL1B) (as WB positive control) | 100 ug |
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|
|
| TP302079 | Recombinant protein of human interleukin 1, beta (IL1B), 20 µg | 20 ug |
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| TP721027 | Purified recombinant protein of Human interleukin 1, beta (IL1B) | 10 ug |
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| TP721175 | Purified recombinant protein of Human interleukin 1, beta (IL1B) | 10 ug |
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|
| TP723772 | Purified recombinant protein of Human interleukin 1, beta (IL1B) | 10 ug |
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|
| TP750014 | Recombinant protein of human IL 1 beta (IL1B) produced in E. coli. | 100 ug |
$540.00
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|
Citations
Prolonged hyperinsulinemia increases the production of inflammatory cytokines in equine digital lamellae but not in striated muscle
Jayathilake, de Laat, Furr et al
Vet J (2023) 303, 106053 DOI: 10.1016/j.tvjl.2023.106053
Jayathilake, de Laat, Furr et al
Vet J (2023) 303, 106053 DOI: 10.1016/j.tvjl.2023.106053
Cited by 2
AI Summary: OriGene's Transient Overexpression Lysate of IL-1β (code LY400196) was used as a positive control for protein expression analysis in this study investigating inflammatory cytokine production in equine tissues during prolonged hyperinsulinemia.
Snippet: Hyperinsulinemia is the key feature of equine metabolic syndrome (EMS) which leads to debilitating sequelae. Hyperinsulinemia-associated laminitis (HAL) is one of the Save;, Transient overexpression lysate of IL-1β (Origene, code LY400196);for IL-6, mouse lung;for TNFα, mouse liver;and for NF-κ B, NF-κB Control cell extracts (Cell signaling, code 9243)
Equine insulin dysregulation causes tissue specific alterations of proinflammatory cytokines and acute phase proteins in a NF-kB independent manner
Jayathilake, Furr, Risco et al
Vet Immunol Immunopathol (2022) 253, 110500 DOI: 10.1016/j.vetimm.2022.110500
Jayathilake, Furr, Risco et al
Vet Immunol Immunopathol (2022) 253, 110500 DOI: 10.1016/j.vetimm.2022.110500
Cited by 6
AI Summary: OriGene's IL1β transient overexpression lysate (product no: LY400196) was used as a positive control for Western blotting to assess IL-1β protein expression in tissue samples in this study.
Snippet: Transient overexpression lysate of IL1 β (ORIGENE, product no: LY400196), IL-6;mouse lung, TNFα;mouse liver, NF-κ B;NF-κB Control Cell Extracts (Cell signaling, product no: 9243). Equal protein loading was confirmed using a housekeeping protein (β actin in adipose tissue and
Showing 1-2 of 2 papers.
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