PGK1 (NM_000291) Human Over-expression Lysate

Snippet: … Lentiviral Overexpression of GAPDH and PGK1 All overexpression plasmids were purchased from Origene as frozen plasmids, all derived from the parental pLenti-C-Myc-DDK backbone (RC202309L1). 293T cells were plated in Poly-L-Lysine-coated 15 cm2 dishes and lipofected using Trans-IT 293 reagent;aqueous fraction was dried and resuspended in 1% acetic acid in water, pH 9 to preserve NAD redox state. LENTIVIRAL OVEREXPRESSION OF GAPDH AND PGK1 All overexpression plasmids were purchased from Origene as frozen plasmids, all derived from the parental pLenti-C-Myc-DDK backbone (RC202309L1). 293T cells were plated in Poly-L-Lysine-coated 15 cm2 dishes and lipofected using Trans-IT 293 reagent;Empty Myc-DDK Lentivirus Plasmid Origene PS100064;http://www.origene.com/catalog/vectors/lentiviral-gene-expression-vectors/ps100064/plenti-c-myc-ddk-lentiviral-gene-expression-vector GAPDH Myc-DDK Lentivirus Plasmid Origene RC202309L1;https://www.origene.com/cDNA_clone/LentiORF/NM_002046/RC202309L1V/GAPDH.aspx PGK1 Myc-DDK Lentivirus Plasmid Origene RC211172L1

Snippet: Full-length, human PGK1 (NM_000291, Origene) transiently expressed from a pCMV6 entry vector with a C-terminal Myc-DDK tag;full-length, human KEAP1 (28023, Addgene) was transiently expressed from a pcDNA/FRT/TO plasmid with a C-terminal 3×Flag tag. All references to Flag–PGK1 or Flag–KEAP1 represent the proteins in the aforementioned vectors, respectively