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NCF1 (NM_000265) Human Over-expression Lysate

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SKU
LY400103
Transient overexpression lysate of neutrophil cytosolic factor 1 (NCF1) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201233
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000265, NP_000256
Synonyms CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A
Predicted MW 44.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000256
Locus ID 653361
Cytogenetics 7q11.23
Protein Families WB
Protein Pathways Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
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Other "NCF1" proteins (4)
SKU Description Size Price
PH301233 NCF1 MS Standard C13 and N15-labeled recombinant protein (NP_000256) 10 ug
$3,360.00
LC400103 Lyophilized NCF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP301233 Recombinant protein of human neutrophil cytosolic factor 1 (NCF1), 20 µg 20 ug
$867.00
TP761217 Purified recombinant protein of Human neutrophil cytosolic factor 1 (NCF1), full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils
Nagarkoti, Dubey, Awasthi et al
Biochim. Biophys. Acta (2018) 1865 (2), 444-454 DOI: 10.1016/j.bbamcr.2017.11.014
Snippet: Co. (MO, USA). NCF1 (NM_000265) Purified Human Protein was obtained from Origene Inc. 2.1. Isolation and purification of neutrophils. Human neutrophils were isolated from peripheral blood, obtained from healthy donors
Dynamin 2 and c-Abl are novel regulators of hyperoxia-mediated NADPH oxidase activation and reactive oxygen species production in caveolin-enriched microdomains of the endothelium
Singleton, Pendyala, Gorshkova et al
J Biol Chem (2009) 284 (50), 34964-75 DOI: 10.1074/jbc.M109.013771
AI Summary: OriGene products contributed to this study by providing human caveolin-1 siRNA (SR304228) and c-Abl siRNA (SR301548), which were used to silence gene expression and investigate the roles of caveolin-1 and c-Abl in hyperoxia-mediated NADPH oxidase activation and ROS production in endothelial cells.
Showing 1-2 of 2 papers.
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