OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

NCF1 (NM_000265) Human Over-expression Lysate

Be the first to review this product
SKU
LC400103
Lyophilized NCF1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA302677 Goat Polyclonal Antibody against NCF1 / p47phox
100 ug
$565.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "NCF1 or NM_000265"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201233
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000265, NP_000256
Synonyms CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A
Predicted MW 44.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000256
Locus ID 653361
Cytogenetics 7q11.23
Protein Families WB
Protein Pathways Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "NCF1" proteins (4)
SKU Description Size Price
PH301233 NCF1 MS Standard C13 and N15-labeled recombinant protein (NP_000256) 10 ug
$3,360.00
LY400103 Transient overexpression lysate of neutrophil cytosolic factor 1 (NCF1) (as WB positive control) 100 ug
$436.00
TP301233 Recombinant protein of human neutrophil cytosolic factor 1 (NCF1), 20 µg 20 ug
$867.00
TP761217 Purified recombinant protein of Human neutrophil cytosolic factor 1 (NCF1), full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils
Nagarkoti, Dubey, Awasthi et al
Biochim. Biophys. Acta (2018) 1865 (2), 444-454 DOI: 10.1016/j.bbamcr.2017.11.014
Snippet: Co. (MO, USA). NCF1 (NM_000265) Purified Human Protein was obtained from Origene Inc. 2.1. Isolation and purification of neutrophils. Human neutrophils were isolated from peripheral blood, obtained from healthy donors
Dynamin 2 and c-Abl are novel regulators of hyperoxia-mediated NADPH oxidase activation and reactive oxygen species production in caveolin-enriched microdomains of the endothelium
Singleton, Pendyala, Gorshkova et al
J Biol Chem (2009) 284 (50), 34964-75 DOI: 10.1074/jbc.M109.013771
AI Summary: OriGene products contributed to this study by providing human caveolin-1 siRNA (SR304228) and c-Abl siRNA (SR301548), which were used to silence gene expression and investigate the roles of caveolin-1 and c-Abl in hyperoxia-mediated NADPH oxidase activation and ROS production in endothelial cells.
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products