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PDX1 (NM_000209) Human Over-expression Lysate

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SKU
LY400074
Transient overexpression lysate of pancreatic and duodenal homeobox 1 (PDX1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222354
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000209, NP_000200
Synonyms GSF; IDX-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; STF-1
Predicted MW 30.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000200
Locus ID 3651
Cytogenetics 13q12.2
Protein Families WB
Protein Pathways Maturity onset diabetes of the young, Type II diabetes mellitus
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Other "PDX1" proteins (4)
SKU Description Size Price
PH322354 PDX1 MS Standard C13 and N15-labeled recombinant protein (NP_000200) 10 ug
$3,360.00
LC400074 Lyophilized PDX1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP322354 Recombinant protein of human pancreatic and duodenal homeobox 1 (PDX1), 20 µg 20 ug
$737.00
TP760660 Purified recombinant protein of Human pancreatic and duodenal homeobox 1 (PDX1), with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Knockdown of PDX1 enhances the osteogenic differentiation of ADSCs partly via activation of the PI3K/Akt signaling pathway
Liu, Chen, Fan
J Orthop Surg Res (2022) 17 (1), 107 DOI: 10.1186/s13018-021-02825-4
Cited by 5
AI Summary: In this study, the PDX1 overexpression plasmid (Flag-PDX1) from OriGene Technologies was used to investigate the role of PDX1 in regulating osteogenic differentiation of adipose-derived stem cells.
Snippet: according to previous literature [15]. The inhibitor efficacy was further identified by western blot to assess the protein level of p-Akt. PDX1 overexpression plasmid (Flag- PDX1) was purchased from Origene Technologies (Origene). Alkaline phosphatase (ALP) activity After treatment, each plate was fixed by 4% paraformaldehyde for 10 min and then washed with PBS for three times. Cells were stain
FigureFigureFigure
Showing 1-1 of 1 papers.
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