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CD1E (NM_001185114) Human Over-expression Lysate

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SKU
LC434131
Lyophilized CD1E HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC231132
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001185114, NP_001172043
Synonyms CD1A; R2
Predicted MW 32.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001172043
Locus ID 913
Cytogenetics 1q23.1
Protein Families WB
Protein Pathways Hematopoietic cell lineage
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Other "CD1E" proteins (12)
SKU Description Size Price
LC403088 Lyophilized CD1E HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC420993 Lyophilized CD1E HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC434141 Lyophilized CD1E HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY434131 Transient overexpression lysate of CD1e molecule (CD1E), transcript variant 13 (as WB positive control) 100 ug
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LY434141 Transient overexpression lysate of CD1e molecule (CD1E), transcript variant 7 (as WB positive control) 100 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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