OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Apolipoprotein E (APOE) (NM_000041) Human Over-expression Lysate

Be the first to review this product
SKU
LC424959
Lyophilized APOE HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA309573 Mouse Monoclonal ApoE4 Antibody (4E4)
100 ul
$620.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "Apolipoprotein E or NM_000041"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200395
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000041, NP_000032
Synonyms AD2; APO-E; ApoE4; LDLCQ5; LPG
Predicted MW 36.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000032
Locus ID 348
Cytogenetics 19q13.32
Protein Families WB
Protein Pathways Alzheimer's disease
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "Apolipoprotein E" proteins (9)
SKU Description Size Price
PH300395 APOE MS Standard C13 and N15-labeled recombinant protein (NP_000032) 10 ug
$3,360.00
LY424959 Transient overexpression lysate of apolipoprotein E (APOE) (as WB positive control) 100 ug
$436.00
TP300395 Recombinant protein of human apolipoprotein E (APOE), 20 µg 20 ug
$737.00
TP721217 Purified recombinant protein of Human apolipoprotein E (APOE) 10 ug
$130.00
TP750215 Purified recombinant protein of Human Apolipoprotein E isoform 1, 19Lys-End, tag free, expressed in E.coli, 50ug 50 ug
$240.00
TP750216 Purified recombinant protein of Human Apolipoprotein E isoform 1, 19Lys-End(126Ala), tag free, expressed in E.coli, 50ug 50 ug
$240.00
TP750217 Purified recombinant protein of Human Apolipoprotein E isoform 1, 19Lys-End(79Ala), tag free, expressed in E.coli, 50ug 50 ug
$240.00
TP750218 Purified recombinant protein of Human Apolipoprotein E isoform 1, 19Lys-End(85Ala), tag free, expressed in E.coli, 50ug 50 ug
$240.00
TP750219 Purified recombinant protein of Human Apolipoprotein E isoform 1, 19Lys-End(75Ala), tag free, expressed in E.coli, 50ug 50 ug
$240.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products