Apolipoprotein E (APOE) Mouse Monoclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, ICC/IF, IP, WB |
|---|---|
| Recommended Dilution | Western Blot: 1:1000-1:2000, Immunoprecipitation: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 2-5 ug/ml, ELISA: 1:100-1:2000 |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1, kappa |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide made to an internal region of human ApoE4 (within residues 100-150). |
| Buffer | Tris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide |
| Concentration | lot specific |
| Purification | Protein G purified |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | apolipoprotein E |
| Database Link | |
| Synonyms | AD2; APO-E; LDLCQ5; LPG |
| Reference Data | |
| Protein Categories | Nervous system: Neurodegenerative diseases, Secreated Proteins |
| Protein Families | Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency |
| Protein Pathways | Alzheimer's disease |
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Citations
ApoE attenuates unresolvable inflammation by complex formation with activated C1q
Yin, Ackermann, Ma et al
Nat. Med. (2019)
Yin, Ackermann, Ma et al
Nat. Med. (2019)
AI Summary: OriGene products, specifically the human APOE expression plasmid (RC208446) and C1QA expression plasmid (RC223883), were used in this study to express and investigate the interactions between ApoE and C1q, helping to elucidate their role in modulating complement activity and inflammation.
Snippet: Cells were incubated in NHS or C1q-depleted human serum (each 1%) (Complement Technology, TX, USA) for 30 min. Cells were blocked and treated with rabbit anti-ApoE antibody (25 µg/ml) (Acris Antibodies, Herford, Germany) and mouse anti-C1q antibody (25 µg/ml) (Thermo Fisher Scientific, MA, USA)
PPARγ and RXR Ligands Disrupt the Inflammatory Cross-talk in the Hypoxic Breast Cancer Stem Cells Niche
Papi, De Carolis, Bertoni et al
J. Cell. Physiol. (2014)
Papi, De Carolis, Bertoni et al
J. Cell. Physiol. (2014)
Cited by 54
AI Summary: OriGene's mouse monoclonal anti-ApoE antibody was used in this study to detect Apolipoprotein E expression, helping assess how nuclear receptor agonists affect cancer stem cell regulatory genes in breast cancer research.
Snippet: Cell lysates were prepared, run, and blotted as previous described (Papi et al., 2012) and probed with specific antibodies: mouse monoclonal anti-ApoE (Origene, Rockville, MD, USA), anti-CAIX (clone M-75, kindly provided by Jaromir Pastorek, Slovak academy of ... Cite SaveSaving
Peroxisome proliferator activated receptor-α/hypoxia inducible factor-1α interplay sustains carbonic anhydrase IX and apoliprotein E expression in breast cancer stem cells
Papi, Storci, Guarnieri et al
PLoS ONE (2013) 8 (1), e54968 DOI: 10.1371/journal.pone.0054968
Papi, Storci, Guarnieri et al
PLoS ONE (2013) 8 (1), e54968 DOI: 10.1371/journal.pone.0054968
Cited by 45
AI Summary: In this study, OriGene products—specifically, PPARα and PPARγ expression plasmids—were used to investigate the role of nuclear receptors in regulating pro-inflammatory pathways and stem cell functions in breast cancer.
Snippet: purchased from Origene (Rockville, MD, USA). CAIX and HIF1 siRNA and appropriate control SCR siRNAs were purchased from Invitrogen (Rockville, MD, USA). Pre-miRNA17-5p, miRNA130b, antago-miRNA17-5p and miRNA130b were purchased from Life Technologies
Showing 1-3 of 3 papers.
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