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Apolipoprotein E (APOE) (NM_000041) Human Tagged ORF Clone

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SKU
RC200395
APOE (Myc-DDK-tagged)-Human apolipoprotein E (APOE)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Apolipoprotein E
Synonyms AD2; APO-E; ApoE4; LDLCQ5; LPG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200395 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTTCTGTGGGCTGCGTTGCTGGTCACATTCCTGGCAGGATGCCAGGCCAAGGTGGAGCAAGCGG
TGGAGACAGAGCCGGAGCCCGAGCTGCGCCAGCAGACCGAGTGGCAGAGCGGCCAGCGCTGGGAACTGGC
ACTGGGTCGCTTTTGGGATTACCTGCGCTGGGTGCAGACACTGTCTGAGCAGGTGCAGGAGGAGCTGCTC
AGCTCCCAGGTCACCCAGGAACTGAGGGCGCTGATGGACGAGACCATGAAGGAGTTGAAGGCCTACAAAT
CGGAACTGGAGGAACAACTGACCCCGGTGGCGGAGGAGACGCGGGCACGGCTGTCCAAGGAGCTGCAGGC
GGCGCAGGCCCGGCTGGGCGCGGACATGGAGGACGTGTGCGGCCGCCTGGTGCAGTACCGCGGCGAGGTG
CAGGCCATGCTCGGCCAGAGCACCGAGGAGCTGCGGGTGCGCCTCGCCTCCCACCTGCGCAAGCTGCGTA
AGCGGCTCCTCCGCGATGCCGATGACCTGCAGAAGCGCCTGGCAGTGTACCAGGCCGGGGCCCGCGAGGG
CGCCGAGCGCGGCCTCAGCGCCATCCGCGAGCGCCTGGGGCCCCTGGTGGAACAGGGCCGCGTGCGGGCC
GCCACTGTGGGCTCCCTGGCCGGCCAGCCGCTACAGGAGCGGGCCCAGGCCTGGGGCGAGCGGCTGCGCG
CGCGGATGGAGGAGATGGGCAGCCGGACCCGCGACCGCCTGGACGAGGTGAAGGAGCAGGTGGCGGAGGT
GCGCGCCAAGCTGGAGGAGCAGGCCCAGCAGATACGCCTGCAGGCCGAGGCCTTCCAGGCCCGCCTCAAG
AGCTGGTTCGAGCCCCTGGTGGAAGACATGCAGCGCCAGTGGGCCGGGCTGGTGGAGAAGGTGCAGGCTG
CCGTGGGCACCAGCGCCGCCCCTGTGCCCAGCGACAATCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200395 protein sequence
Red=Cloning site Green=Tags(s)

MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELL
SSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEV
QAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRA
ATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLK
SWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000041
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000041.4
RefSeq Size 1223 bp
RefSeq ORF 954 bp
Locus ID 348
UniProt ID P02649
Cytogenetics 19q13.32
Domains Apolipoprotein
Protein Families Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency
Protein Pathways Alzheimer's disease
MW 36.2 kDa
Summary The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. provided by RefSeq, Jun 2016
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"NM_000041" in other vectors (6)
SKU Description Size Price
RC200395L1 Lenti ORF clone of Human apolipoprotein E (APOE), Myc-DDK-tagged 10 ug
$600.00
RC200395L2 Lenti ORF clone of Human apolipoprotein E (APOE), mGFP tagged 10 ug
$600.00
RC200395L3 Lenti ORF clone of Human apolipoprotein E (APOE), Myc-DDK-tagged 10 ug
$600.00
RC200395L4 Lenti ORF clone of Human apolipoprotein E (APOE), mGFP tagged 10 ug
$600.00
RG200395 APOE (tGFP-tagged) - Human apolipoprotein E (APOE) 10 ug
$489.00 $500.00
SC319433 APOE (untagged)-Human apolipoprotein E (APOE) 10 ug
$300.00

Plasmid Map for RC200395

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200395
Circular map for RC200395

file-alt Citations

Isoform-dependent lysosomal degradation and internalization of apolipoprotein E requires autophagy proteins
Fote, Geller, Efstathiou et al
J Cell Sci (2022) 135 (2) DOI: 10.1242/jcs.258687
Cited by 35
AI Summary: OriGene's APOE3–Myc–Flag plasmid (catalog number RC200395) was used as a template to generate APOE isoform constructs, enabling the study of isoform-specific effects on lysosomal degradation and internalization of apolipoprotein E in this research.
Snippet: …APOE3–Myc–Flag was purchased from Origene (catalog number RC200395). APOE4–Myc–Flag and APOE2–Myc–Flag were generated through mutagenesis from APOE3–Myc–flag, APOE3–mCh–SepHluorin and APOE3–mCh–SepHluorin was gener;APOE3–Myc–Flag was purchased from Origene (catalog number RC200395)
SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE
Raihan, Brishti, Li et al
Cell Rep (2019) 28 (1), 78-90.e6 DOI: 10.1016/j.celrep.2019.06.002
AI Summary: In this study, the OriGene product "apoE3 (human), catalog number RC200395," was used to restore ApoE expression in experimental models, helping to demonstrate that replenishing ApoE can rescue aging-like cognitive deficits caused by SFRS11 loss.
Snippet: Skip to article;pmirGlo-LRP8 (S1 mutation) This Paper N/A pmirGlo-ApoE (S1 mutation) This Paper N/A Human SFRS11 shRNA Lee et al., 2015 SFRS11-1 pLenti puro Addgene 39481 pAAV-EF1a-DIO-Gcamp6s Addgene 67526 apoE3 (human) Origene RC200395
Human ApoE Isoforms Differentially Modulate Brain Glucose and Ketone Body Metabolism: Implications for Alzheimer's Disease Risk Reduction and Early Intervention
Wu, Zhang, Zhao
J. Neurosci. (2018) 38 (30), 6665-6681 DOI: 10.1523/JNEUROSCI.2262-17.2018
AI Summary: OriGene products contributed to this study by providing the human ApoE3 cDNA clone (NM_000041) in the pCMV6-Entry vector with a C-terminal Myc-DDK tag, which was directly purchased, and by serving as the template for generating genetically modified ApoE2 and ApoE4 cDNA clones used to investigate the effects of ApoE isoforms on brain energy metabolism.
Snippet: Human ApoE2, ApoE3, and ApoE4 cDNA clones expressed in the mammalian vector pCMV6 with C-terminal Myc-DDK Tag were procured from OriGene Technologies. ApoE3 cDNA clone (NM_000041) was directly purchased from OriGene. ApoE2 and ApoE4 cDNA clones were generated by genetic modification of ApoE3 cDNA sequence. Specifically, ApoE2 cDNA clone was obtained by mutation on nucleotide position 388 T to C
Showing 1-3 of 3 papers.
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