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ICAM2 (NM_001099787) Human Over-expression Lysate

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SKU
LC420536
Lyophilized ICAM2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212720
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001099787, NP_001093257
Synonyms CD102
Predicted MW 30.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001093257
Locus ID 3384
Cytogenetics 17q23.3
Protein Families WB
Protein Pathways Cell adhesion molecules (CAMs), Natural killer cell mediated cytotoxicity
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Other "ICAM2" proteins (19)
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PH300459 ICAM2 MS Standard C13 and N15-labeled recombinant protein (NP_000864) 10 ug
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PH312720 ICAM2 MS Standard C13 and N15-labeled recombinant protein (NP_001093257) 10 ug
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PH316706 ICAM2 MS Standard C13 and N15-labeled recombinant protein (NP_001093258) 10 ug
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LC420535 Lyophilized ICAM2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420537 Lyophilized ICAM2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420538 Lyophilized ICAM2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC424476 Lyophilized ICAM2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC426090 Lyophilized ICAM2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY420535 Transient overexpression lysate of intercellular adhesion molecule 2 (ICAM2), transcript variant 1 (as WB positive control) 100 ug
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LY420536 Transient overexpression lysate of intercellular adhesion molecule 2 (ICAM2), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY420537 Transient overexpression lysate of intercellular adhesion molecule 2 (ICAM2), transcript variant 3 (as WB positive control) 100 ug
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LY420538 Transient overexpression lysate of intercellular adhesion molecule 2 (ICAM2), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY424476 Transient overexpression lysate of intercellular adhesion molecule 2 (ICAM2), transcript variant 5 (as WB positive control) 100 ug
$436.00
TP300459 Recombinant protein of human intercellular adhesion molecule 2 (ICAM2), transcript variant 5, 20 µg 20 ug
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TP312720 Purified recombinant protein of Homo sapiens intercellular adhesion molecule 2 (ICAM2), transcript variant 2, 20 µg 20 ug
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TP312775 Purified recombinant protein of Homo sapiens intercellular adhesion molecule 2 (ICAM2), transcript variant 4, 20 µg 20 ug
$867.00
TP316706 Purified recombinant protein of Homo sapiens intercellular adhesion molecule 2 (ICAM2), transcript variant 3, 20 µg 20 ug
$867.00
TP720626 Purified recombinant protein of Human intercellular adhesion molecule 2 (ICAM2), transcript variant 5 10 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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