GAPDH (NM_002046) Human Over-expression Lysate
SKU
LC419567
Lyophilized GAPDH HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC202309 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002046, NP_002037 |
| Synonyms | G3PD; GAPD; HEL-S-162eP |
| Predicted MW | 36.1 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_002037 |
| Locus ID | 2597 |
| Cytogenetics | 12p13.31 |
| Protein Families | WB |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "GAPDH" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH302309 | GAPDH MS Standard C13 and N15-labeled recombinant protein (NP_002037) | 10 ug |
$3,360.00
|
|
| LY419567 | Transient overexpression lysate of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (as WB positive control) | 100 ug |
$436.00
|
|
| TP302309 | Recombinant protein of human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 20 µg | 20 ug |
$867.00
|
|
Citations
High throughput discovery of functional protein modifications by Hotspot Thermal Profiling
Huang, Lee, Cavanaugh et al
Nat. Methods (2019) 16 (9), 894-901 DOI: 10.1038/s41592-019-0499-3
Huang, Lee, Cavanaugh et al
Nat. Methods (2019) 16 (9), 894-901 DOI: 10.1038/s41592-019-0499-3
AI Summary: OriGene’s full-length Myc-DDK-tagged human vinculin (NM_003373) was used as the template to generate mutant proteins for functional analysis in this study.
Snippet: Full-length Myc-DDK-tagged-human vinculin (NM_003373, Origene) was used to generate mutants with PCR primers in Supplementary Table 3 according to the QuikChange II Site-Directed Mutagenesis Kit manufacturer protocol (200523, Agilent). HeLa cells were grown to 70% confluency in 6-well plates before transfection of 500 ng plasmid with Lipofectamine 2000 (Invitrogen)
Showing 1-1 of 1 papers.
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