GAPDH (NM_002046) Human Recombinant Protein
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 35.9 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_002037 |
| Locus ID | 2597 |
| UniProt ID | P04406 |
| Cytogenetics | 12p13.31 |
| RefSeq Size | 1421 |
| RefSeq ORF | 1005 |
| Synonyms | G3PD; GAPD; HEL-S-162eP |
| Summary | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2014 |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "GAPDH" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH302309 | GAPDH MS Standard C13 and N15-labeled recombinant protein (NP_002037) | 10 ug |
$3,360.00
|
|
| LC419567 | Lyophilized GAPDH HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY419567 | Transient overexpression lysate of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
The role of autoproteolysis and mitoribosomal proteins in regulation of mitochondrial LACTB tumor suppressor
Escudeiro-Lopes, González-Morena, Baudysova et al
bioRxiv (2025)
Escudeiro-Lopes, González-Morena, Baudysova et al
bioRxiv (2025)
AI Summary: OriGene contributed to this study by providing recombinant proteins for MRPS34 (TP300834), GAPDH (TP302309), COQ6 (TP760736), HSBP1 (TP301399), ASCL3 (TP320042), and TOMM20 (TP310746), which were used to investigate the molecular mechanisms regulating the mitochondrial tumor suppressor LACTB.
Identifying G-quadruplex-interacting proteins in cancer-related gene promoters
Marzano, Pinto, Di Porzio et al
Commun Chem (2025) 8 (1), 64 DOI: 10.1038/s42004-025-01462-w
Marzano, Pinto, Di Porzio et al
Commun Chem (2025) 8 (1), 64 DOI: 10.1038/s42004-025-01462-w
Cited by 14
AI Summary: OriGene products—specifically recombinant AHNAK (TP315337), GAPDH (TP302309), and LMNA (TP304970) proteins—were used to validate direct interactions with G-quadruplex DNA structures via surface plasmon resonance experiments in this study.
Snippet: s matrix. Surface plasmon resonance (SPR) experiments SPR experiments were performed at 25 °C using a Biacore X100 (GE Healthcare) equipped with a research-grade CM5 sensor chip. AHNAK (TP315337, OriGene Technologies GmbH, Germany), GAPDH (TP302309, OriGene Technologies GmbH), HNRNP M (orb1784775, Biorbyt, UK), LMNA protein (TP304970, OriGene Technologies GmbH), and PPIA (orb706916, Biorbyt) w
The Effect of Neddylation Inhibition on Inflammation-Induced MMP9 Gene Expression in Esophageal Squamous Cell Carcinoma
Hryniewicz-Jankowska, Wierzbicki, Tabola et al
Int J Mol Sci (2021) 22 (4) DOI: 10.3390/ijms22041716
Hryniewicz-Jankowska, Wierzbicki, Tabola et al
Int J Mol Sci (2021) 22 (4) DOI: 10.3390/ijms22041716
Cited by 10
AI Summary: OriGene's GAPDH primers (HP100003) were used in this study to normalize gene expression levels during real-time PCR analysis.
Snippet: To normalize gene expression, primers for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (OriGene, HP100003) were used
Efficacy of Obcordata A from Aspidopterys obcordata on Kidney Stones by Inhibiting NOX4 Expression
Li, Ma, Lv et al
Molecules (2019) 24 (10) DOI: 10.3390/molecules24101957
Li, Ma, Lv et al
Molecules (2019) 24 (10) DOI: 10.3390/molecules24101957
AI Summary: OriGene products contributed to this study by providing antibodies against NOX4, P38, JNK, OPN, MCP-1, and GAPDH, which were used for immunoblotting and protein expression analysis.
Snippet: 4. Materials and Methods. 4.1. Antibodies and Reagents. The antibodies against NOX4, P38, JNK, OPN, MCP-1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from OriGene Technologies (Rockville, MD, USA) Cached;
Anti-GAPDH Autoantibody Is Associated with Increased Disease Activity and Intracranial Pressure in Systemic Lupus Erythematosus
Sun, Li, Zhou et al
J Immunol Res (2019) 2019, 7430780 DOI: 10.1155/2019/7430780
Sun, Li, Zhou et al
J Immunol Res (2019) 2019, 7430780 DOI: 10.1155/2019/7430780
AI Summary: OriGene's recombinant human GAPDH protein was used to coat ELISA plates, enabling the detection and measurement of anti-GAPDH autoantibodies in patient serum samples in this study.
Snippet: as previously described [16]. Briefly, 96-well polysorp plates (Nunc, Denmark) were coated with recombinant human GAPDH protein (OriGene, Beijing, China) of 1 μg/mL in carbonate buffer at 4°C overnight. The wells were Cached
In vitro evaluation of a basic fibroblast growth factor-containing hydrogel toward vocal fold lamina propria scar treatment
Erndt-Marino, Jimenez-Vergara, Diaz-Rodriguez et al
J. Biomed. Mater. Res. Part B Appl. Biomater. (2017)
Erndt-Marino, Jimenez-Vergara, Diaz-Rodriguez et al
J. Biomed. Mater. Res. Part B Appl. Biomater. (2017)
AI Summary: OriGene products, including primers or reagents for iNOS, TNF, IL-12β, Arg-1, IL-10, Chi3l3, and GAPDH, were used to assess gene expression changes in macrophages and fibroblasts, enabling evaluation of the hydrogel's anti-fibrotic and anti-inflammatory effects.
Snippet: nitric oxide synthase (iNOS), tumor necrosis factor (TNF), subunit beta of interleukin 12 (IL-12β), arginase-1 (Arg-1), interleukin-10 (IL-10), chitinase-3 like protein 3 (Chi3l3), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Origene or
Showing 1-6 of 8 papers.
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