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GAPDH (NM_002046) Human Tagged ORF Clone

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SKU
RC202309
GAPDH (Myc-DDK-tagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "GAPDH or NM_002046"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GAPDH
Synonyms G3PD; GAPD; HEL-S-162eP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202309 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTCACCAGGGCTGCTTTTA
ACTCTGGTAAAGTGGATATTGTTGCCATCAATGACCCCTTCATTGACCTCAACTACATGGTTTACATGTT
CCAATATGATTCCACCCATGGCAAATTCCATGGCACCGTCAAGGCTGAGAACGGGAAGCTTGTCATCAAT
GGAAATCCCATCACCATCTTCCAGGAGCGAGATCCCTCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGT
ACGTCGTGGAGTCCACTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAA
AAGGGTCATCATCTCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTGTGAACCATGAGAAGTAT
GACAACAGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAGCACCCCTGGCCAAGGTCA
TCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCCATCACTGCCACCCAGAAGAC
TGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGCGGGGCTCTCCAGAACATCATCCCTGCCTCT
ACTGGCGCTGCCAAGGCTGTGGGCAAGGTCATCCCTGAGCTGAACGGGAAGCTCACTGGCATGGCCTTCC
GTGTCCCCACTGCCAACGTGTCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGA
CATCAAGAAGGTGGTGAAGCAGGCGTCGGAGGGCCCCCTCAAGGGCATCCTGGGCTACACTGAGCACCAG
GTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTCCACCTTTGACGCTGGGGCTGGCATTGCCCTCA
ACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGGTGGACCT
CATGGCCCACATGGCCTCCAAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202309 protein sequence
Red=Cloning site Green=Tags(s)

MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVIN
GNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKY
DNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPAS
TGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQ
VVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002046
ORF Size 1005 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002046.7
RefSeq Size 1421 bp
RefSeq ORF 1008 bp
Locus ID 2597
UniProt ID P04406
Cytogenetics 12p13.31
Domains gpdh
Protein Families ES Cell Differentiation/IPS
Protein Pathways Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways
MW 36.1 kDa
Summary This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2014
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Documents
Resources
"NM_002046" in other vectors (6)
SKU Description Size Price
RC202309L1 Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged 10 ug
$986.00
RC202309L2 Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged 10 ug
$986.00
RC202309L3 Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged 10 ug
$986.00
RC202309L4 Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged 10 ug
$986.00
RG202309 GAPDH (tGFP-tagged) - Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 10 ug
$489.00 $886.00
SC118869 GAPDH (untagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 10 ug
$457.00

Plasmid Map for RC202309

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202309
Circular map for RC202309

file-alt Citations

Antitumor Activity of Asperphenin B by Induction of Apoptosis and Regulation of Glyceraldehyde-3-phosphate Dehydrogenase in Human Colorectal Cancer Cells
Byun, Bae, Park et al
J Nat Prod (2021) 84 (3), 683-693 DOI: 10.1021/acs.jnatprod.0c01155
Cited by 13
AI Summary: OriGene products, specifically the GAPDH siRNA (SR304040) and GAPDH overexpression plasmid (RC200963), were used in this study to manipulate GAPDH expression in colorectal cancer cells, enabling the authors to elucidate the role of GAPDH in mediating the antitumor and antimetastatic effects of Asperphenin B.
GAPDH controls extracellular vesicle biogenesis and enhances therapeutic potential of EVs in silencing the Huntingtin gene in mice via siRNA delivery
Dar, Mendes, Kuan et al
bioRxiv (2020)
Cited by 4
Snippet: 1:500 Purification of GAPDH and G58T fusion proteins from bacterial cells. (a) GAPDH protein The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). At the N-terminus of GAPDH, six residues of histidine were inserted for
Malonylation of GAPDH is an inflammatory signal in macrophages
Galván-Peña, Carroll, Newman et al
Nat Commun (2019) 10 (1), 338 DOI: 10.1038/s41467-018-08187-6
AI Summary: OriGene’s myc-DKK-GAPDH plasmid (RC202309) was used as the template for mutagenesis and as the wild-type control to study the role of GAPDH malonylation in macrophage inflammatory signaling.
Snippet: TGGTGCTGCCCAGGCTGTGGGCAAGG GAPDH K213Q R: GATGACCACGACGGGTCCGACACCCGTTCC GAPDH K213E F: CTACTGGTGCTGCCGAGGCTGTGGGCAAGG GAPDH K213E R: GATGACCACGACGGCTCCGACACCCGTTCC The myc-DKK-GAPDH plasmid from Origene Technologies (Rockville, MD) (RC202309) was used as the template for mutagenesis and as the wild-type in experiments. psi-CHECK2 plasmid (Promega) was used for luciferase assays. Luciferase a
FigureFigureFigure
GAPDH with NAD+-binding site mutation competitively inhibits the wild-type and affects glucose metabolism in cancer
Kunjithapatham, Ganapathy-Kanniappan
Biochim. Biophys. Acta (2018)
Snippet: 2. Materials and methods. 2.1. Chemicals, reagents and cell lines. Unless otherwise indicated all chemicals were purchased from Sigma Chemical Co., (St Louis, MO, USA). Human GAPDH clone with myc-DDK tag was procured from OriGene Technologies (Rockville
Protein CoAlation: A Redox-Regulated Protein Modification by Coenzyme Ain Mammalian Cells
Tsuchiya, Peak-Chew, Newell et al
Biochem. J. (2017)
AI Summary: OriGene’s pCMV6-Entry vectors encoding FLAG-tagged full-length human GAPDH were used for transient transfection in HEK293 cells, enabling the study of protein CoAlation and its regulatory effects in mammalian cells.
Snippet: Transient transfection and immunoprecipitation HEK293 and HEK293/Pank1β cells were transfected with Origene's pCMV6-Entry vectors encoding FLAG-tagged full-length human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), ... Cite SaveSaving
Overexpression of glyceraldehyde 3-phosphate dehydrogenase prevents neurovascular degeneration after retinal injury
Cai, Xue, Liu et al
FASEB J. (2015)
Cited by 30
AI Summary: OriGene Technologies contributed to this study by providing the human GAPDH (hGAPDH)-ORF product, which was used to overexpress GAPDH in cell lines to investigate its protective role against neurovascular degeneration after retinal injury.
Snippet: Jiangsu, China). Briefly, the human GAPDH (hGAPDH)-ORF, purchased from OriGene Technologies (Rockville, MD, USA), was amplified by PCR, and the resulting product was digested with EcoRI and Sacl, and pu- rified. The ... Cite SaveSaving
Showing 1-6 of 7 papers.
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