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GAPDH (NM_002046) Human Tagged ORF Clone Lentiviral Particle

  • LentiORF®
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SKU
RC202309L1V
Lenti ORF particles, GAPDH (Myc-DDK tagged) - Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 200ul, >10^7 TU/mL
  $1,236.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone Lentiviral Particle
Tag Myc-DDK
Target Symbol GAPDH
Synonyms G3PD; GAPD; HEL-S-162eP
Vector pLenti-C-Myc-DDK
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC202309).
ACCN NM_002046
ORF Size 1005 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Shipping Dry Ice
Reference Data
RefSeq NM_002046.2
RefSeq Size 1421 bp
RefSeq ORF 1008 bp
Locus ID 2597
UniProt ID P04406
Cytogenetics 12p13.31
Domains gpdh
Protein Families ES Cell Differentiation/IPS
Protein Pathways Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways
MW 36.1 kDa
Summary This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2014
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Documents
Resources

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Glucose Response by Stem Cell-Derived β Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis
Davis, Alves, Helman et al
Cell Rep (2020) 31 (6), 107623 DOI: 10.1016/j.celrep.2020.107623
Cited by 145
AI Summary: OriGene contributed to this study by providing the pLenti-C-Myc-DDK lentiviral gene expression vector (PS100064), GAPDH Myc-DDK lentivirus plasmid (RC202309L1), and PGK1 Myc-DDK lentivirus plasmid (RC211172L1) used for overexpression experiments to investigate glycolytic bottlenecks in stem cell-derived β cells.
Snippet: … Lentiviral Overexpression of GAPDH and PGK1 All overexpression plasmids were purchased from Origene as frozen plasmids, all derived from the parental pLenti-C-Myc-DDK backbone (RC202309L1). 293T cells were plated in Poly-L-Lysine-coated 15 cm2 dishes and lipofected using Trans-IT 293 reagent;aqueous fraction was dried and resuspended in 1% acetic acid in water, pH 9 to preserve NAD redox state. LENTIVIRAL OVEREXPRESSION OF GAPDH AND PGK1 All overexpression plasmids were purchased from Origene as frozen plasmids, all derived from the parental pLenti-C-Myc-DDK backbone (RC202309L1). 293T cells were plated in Poly-L-Lysine-coated 15 cm2 dishes and lipofected using Trans-IT 293 reagent;Empty Myc-DDK Lentivirus Plasmid Origene PS100064;http://www.origene.com/catalog/vectors/lentiviral-gene-expression-vectors/ps100064/plenti-c-myc-ddk-lentiviral-gene-expression-vector GAPDH Myc-DDK Lentivirus Plasmid Origene RC202309L1;https://www.origene.com/cDNA_clone/LentiORF/NM_002046/RC202309L1V/GAPDH.aspx PGK1 Myc-DDK Lentivirus Plasmid Origene RC211172L1
MicroRNA Let-7f-1-3p attenuates smoke-induced apoptosis in bronchial and alveolar epithelial cells in vitro by targeting FOXO1
Han, Zhu, Cui et al
Eur. J. Pharmacol. (2019) 862, 172531 DOI: 10.1016/j.ejphar.2019.172531
AI Summary: OriGene contributed to this study by providing the qPCR primer pairs for FOXO1 (NM_002015) and GAPDH (NM_002046), which were used to measure gene expression levels in bronchial and alveolar epithelial cells.
Snippet: following manufacturer's instructions. GAPDH was used as reference gene for FOXO1. FOXO1 (NM_002015) and GAPDH (NM_002046) qPCR primer pairs were purchased from OriGene (Rockville, MD, USA). For Western blot
Glyceraldehyde-3-phosphate dehydrogenase acts as a mitochondrial trans-s-nitrosylase in the heart
Kohr, Murphy, Steenbergen
PLoS ONE (2014) 9 (10), e111448 DOI: 10.1371/journal.pone.0111448
Cited by 49
AI Summary: OriGene contributed to this study by providing the DDK-tagged human GAPDH protein, which was used to investigate the role of GAPDH as a mitochondrial trans-S-nitrosylase in heart tissue.
Snippet: DDK-tagged human GAPDH was purchased from Origene (Rockville, MD), and mutation of C150 to a serine residue was performed using the QuickChange II site-directed mutagenesis kit (Agilent, Santa Clara, CA) according to the manufacturer's instruction. ... Cite SaveSaving
Showing 1-3 of 3 papers.
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