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HES1 (NM_005524) Human Over-expression Lysate

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SKU
LC417251
Lyophilized HES1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211709
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005524, NP_005515
Synonyms bHLHb39; HES-1; HHL; HRY
Predicted MW 29.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005515
Locus ID 3280
Cytogenetics 3q29
Protein Families WB
Protein Pathways Maturity onset diabetes of the young, Notch signaling pathway
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Other "HES1" proteins (4)
SKU Description Size Price
PH311709 HES1 MS Standard C13 and N15-labeled recombinant protein (NP_005515) 10 ug
$3,360.00
LY417251 Transient overexpression lysate of hairy and enhancer of split 1, (Drosophila) (HES1) (as WB positive control) 100 ug
$436.00
TP311709 Recombinant protein of human hairy and enhancer of split 1, (Drosophila) (HES1), 20 µg 20 ug
$737.00
TP760671 Purified recombinant protein of Human hairy and enhancer of split 1, (Drosophila) (HES1), with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway
Xie, Sun, Chen et al
Oxid Med Cell Longev (2015) 2015, 214836 DOI: 10.1155/2015/214836
Cited by 65
AI Summary: In this study, OriGene’s Notch-1 siRNA (SR321712) was used to silence Notch-1 expression in HUVEC cells, enabling the researchers to demonstrate that EGCG reduces uric acid-induced inflammation and oxidative stress through modulation of the NOTCH signaling pathway.
FigureFigureFigure
Suppression of p53 by Notch3 is mediated by Cyclin G1 and sustained by MDM2 and miR-221 axis in hepatocellular carcinoma
Giovannini, Minguzzi, Baglioni et al
Oncotarget (2014) 5 (21), 10607-20 DOI: 10.18632/oncotarget.2523
Cited by 45
AI Summary: In this study, OriGene’s Notch3 shRNA (TG313586) and MDM2 shRNA (TG313386) were used to silence gene expression and investigate the regulatory relationship between Notch3, p53, Cyclin G1, MDM2, and miR-221 in hepatocellular carcinoma cells.
FigureFigureFigure
Showing 1-2 of 2 papers.
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