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CD133 (PROM1) (NM_006017) Human Over-expression Lysate

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SKU
LC416886
Lyophilized PROM1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221611
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006017, NP_006008
Synonyms AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4
Predicted MW 97 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006008
Locus ID 8842
Cytogenetics 4p15.32
Protein Families WB
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Other "CD133" proteins (7)
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PH321611 PROM1 MS Standard C13 and N15-labeled recombinant protein (NP_006008) 10 ug
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LC429028 Lyophilized PROM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY416886 Transient overexpression lysate of prominin 1 (PROM1), transcript variant 1 (as WB positive control) 100 ug
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LY429028 Transient overexpression lysate of prominin 1 (PROM1), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP321611 Recombinant protein of human prominin 1 (PROM1), transcript variant 1, 20 µg 20 ug
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TP327854 Recombinant protein of human prominin 1 (PROM1), transcript variant 2, 20 µg 20 ug
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file-alt Citations

Acidic Fibroblast Growth Factor Promotes Endothelial Progenitor Cells Function via Akt/FOXO3a Pathway
Huang, Wang, Wang et al
PLoS ONE (2015) 10 (6), e0129665 DOI: 10.1371/journal.pone.0129665
Cited by 13
AI Summary: In this study, the CD133 antibody from OriGene was used to immunofluorescently identify endothelial progenitor cells, enabling the researchers to assess the effects of FGF1 on EPC function.
Snippet: experiments. For EPC identification, cells were immunofluorescently stained using antibodies against endothelial markers CD34 (Epitomics) and VEGFR-2 (R&D Systems) as well as stem/progenitor marker CD133 (Origene). ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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