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TFEB (NM_007162) Human Over-expression Lysate

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SKU
LC416167
Lyophilized TFEB HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207153
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_007162, NP_009093
Synonyms ALPHATFEB; BHLHE35; TCFEB
Predicted MW 52.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_009093
Locus ID 7942
Cytogenetics 6p21.1
Protein Families WB
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Other "TFEB" proteins (2)
SKU Description Size Price
LY416167 Transient overexpression lysate of transcription factor EB (TFEB), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP760282 Recombinant protein of human transcription factor EB (TFEB), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

PGC-1α rescues Huntington's disease proteotoxicity by preventing oxidative stress and promoting TFEB function
Tsunemi, Ashe, Morrison et al
Sci Transl Med (2012) 4 (142), 142ra97 DOI: 10.1126/scitranslmed.3003799
Cited by 442
AI Summary: OriGene products contributed to this study by providing the human PGC-1α cDNA clone (OriGene, Cat# SC118083), which was used to restore PGC-1α expression and investigate its therapeutic effects on Huntington's disease models.
Snippet: Page 1. DOI: 10.1126/scitranslmed.3003799 , 142ra97 (2012);4 Sci Transl Med et al. Taiji Tsunemi Oxidative Stress and Promoting TFEB Function Rescues Huntington's Disease Proteotoxicity by Preventing α PGC-1 Editor's Summary disorders as well.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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