TFEB (NM_007162) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TFEB |
| Synonyms | ALPHATFEB; BHLHE35; TCFEB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007162 |
| ORF Size | 1428 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007162.2 |
| RefSeq Size | 2364 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 7942 |
| UniProt ID | P19484 |
| Cytogenetics | 6p21.1 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 52.9 kDa |
| Summary | Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007162" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207153L1 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC207153L2 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC207153L3 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC207153L4 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG207153 | TFEB (tGFP-tagged) - Human transcription factor EB (TFEB), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
| SC122773 | TFEB (untagged)-Human transcription factor EB (TFEB), transcript variant 1 | 10 ug |
$686.00
|
|
Citations
Vascular smooth muscle cell-derived hydrogen sulfide promotes atherosclerotic plaque stability via TFEB (transcription factor EB)-mediated autophagy
Chen, Ouyang, Zhang et al
Autophagy (2022) 18 (10), 2270-2287 DOI: 10.1080/15548627.2022.2026097
Chen, Ouyang, Zhang et al
Autophagy (2022) 18 (10), 2270-2287 DOI: 10.1080/15548627.2022.2026097
Cited by 74
Autophagic flux inhibition and lysosomogenesis ensuing cellular capture and retention of the cationic drug quinacrine in murine models
Parks, Charest-Morin, Boivin-Welch et al
PeerJ (2015) 3, e1314 DOI: 10.7717/peerj.1314
Parks, Charest-Morin, Boivin-Welch et al
PeerJ (2015) 3, e1314 DOI: 10.7717/peerj.1314
Cited by 25
AI Summary: OriGene products, specifically the anti-LAMP1 (Cat# TA602382) and anti-LAMP2 (Cat# TA809144) antibodies, were used in this study to detect and quantify lysosomal proteins by immunoblotting, thereby helping to demonstrate increased lysosomogenesis in quinacrine-treated fibroblasts and mouse tissues.
Showing 1-2 of 2 papers.
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