TFEB (NM_007162) Human Recombinant Protein

Snippet: recombinant AMPK was pre-incubated with 0.2 µM BAY-3827 or 0.2 µM BAY-974 for 30 min and subsequently with 1 µM MK-8722 for 30 min. 250 ng recombinant TFEB (OriGene, TP760282) or recombinant ACC2 (Aviva, OPCD00449) was added to the reaction. The kinase reaction was performed in buffer A (50 mM HEPES [Sigma, H3375]

Snippet: 48 h, the cells from all the experimental groups were lysed in CHAPS lysis buffer, followed by pulldown with anti-GFP magnetic beads. The AKT2 complex was incubated with recombinant TFEB (TP760282, Origene) for 15 min at 37 °C in the presence of 10 nm ATP (A1852-1VL, Sigma Aldrich). The mixture was denatured using LDS sample buffer (NP0007, Invitrogen) containing β-marcaptoethanol and th

Snippet: The AKT2 complex was incubated 451 with recombinant TFEB (TP760282, Origene) for 15 minutes at 37oC in the presence of 10 nm 452 ATP (A1852-1VL, Sigma Aldrich)

Snippet: Anti-TFEB (13372-1-AP) was purchased from Proteintech. Anti-TFEB (A303-673A) was purchased from Bethyl Laboratories, Inc. Mouse Atg5 siRNA (L-064838-00-0005), Becn1 siRNA (L-055895-00-0005), Tfeb siRNA (L-050607-02-0005) and nontarget siRNA were purchased from Dharmacon. … SQSTM1 plasmid (MYC-DDK-tagged) (RC203214) and recombinant human TFEB protein (TP760282) were purchased from OriGene Technologies, Inc. …HeLa cells stably expressing 3x-Flag-TFEB5,6 were maintained in DMEM supplemented with 10% FBS and 500 μg/ml G418. …