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NUP50 (NM_007172) Human Over-expression Lysate

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SKU
LC416145
Lyophilized NUP50 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209975
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_007172, NP_009103
Synonyms NPAP60; NPAP60L
Predicted MW 50.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_009103
Locus ID 10762
Cytogenetics 22q13.31
Protein Families WB
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Other "NUP50" proteins (7)
SKU Description Size Price
PH307001 NUP50 MS Standard C13 and N15-labeled recombinant protein (NP_705931) 10 ug
$3,360.00
PH309975 NUP50 MS Standard C13 and N15-labeled recombinant protein (NP_009103) 10 ug
$3,360.00
LC407000 Lyophilized NUP50 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY407000 Transient overexpression lysate of nucleoporin 50kDa (NUP50), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY416145 Transient overexpression lysate of nucleoporin 50kDa (NUP50), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP307001 Recombinant protein of human nucleoporin 50kDa (NUP50), transcript variant 3, 20 µg 20 ug
$737.00
TP309975 Recombinant protein of human nucleoporin 50kDa (NUP50), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

A naturally monomeric infrared fluorescent protein for protein labeling in vivo
Yu, Baird, Allen et al
Nat. Methods (2015)
Cited by 182
AI Summary: In this study, OriGene provided cDNA clones for human lasp1 (NM_006148.3), human myotilin (NM_006790.2), rat sEpsin (NM_019585.3), and human vinculin (NM_003373.3), which were used as templates to generate fusion constructs for testing the new monomeric infrared fluorescent protein (mIFP) in protein labeling experiments.
Snippet: … Patterson, NIH;NM_021058.3);human lamin A/C (30), NheI and BglII (cDNA source: D. Gilbert, Florida State University;NM_170707.2);human lasp1 (22) NheI and BglII (cDNA source: OriGene;NM_006148.3);human myotilin (26), AgeI and BspEI (cDNA source: OriGene;NM_006790.2);human Rab4a (19), BglII and BamHI (cDNA source: V. Allen, University of Manchester;NM_004578.3);rat sEpsin (30) NheI and BglII (cDNA source: OriGene;NM_019585.3);human α-tubulin (30), NheI and BglII (cDNA source: Clontech;NM_006082);human vinculin (35) NheI and EcoRI (cDNA source: OriGene;NM_003373.3)
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
Shaner, Lambert, Chammas et al
Nat. Methods (2013) 10 (5), 407-9 DOI: 10.1038/nmeth.2413
Cited by 1516
AI Summary: OriGene contributed mouse β-catenin cDNA (product: NM_001165902.1) used for cloning and expression experiments in the study.
Snippet: routed by a beam-splitter through the 530/43 nm (acceptor emission) and the 480/40 nm (donor emission) band-pass emission ... NM_001101.3);β-catenin (20 aa), coding sequence cloned between XhoI and BamHI (mouse β-catenin;cDNA source: Origene;NM_001165902.1
Showing 1-2 of 2 papers.
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