OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

PNS-Tau Human Over-expression Lysate 

Be the first to review this product
SKU
LC413820
Lyophilized tau (MAPT), transcript variant 1 (PNS-Tau/Big Tau) HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA809201 MAPT mouse monoclonal antibody,clone OTI13B5 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "Tau or NM_016835"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216166
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_016835, NP_058519
Synonyms DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; tau-40
Predicted MW 78.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_058519
Locus ID 4137
Cytogenetics 17q21.31
Protein Families WB
Protein Pathways Alzheimer's disease, MAPK signaling pathway
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "Tau" proteins (16)
SKU Description Size Price
PH313312 MAPT MS Standard C13 and N15-labeled recombinant protein (NP_005901) 10 ug
$3,360.00
PH313364 MAPT MS Standard C13 and N15-labeled recombinant protein (NP_058525) 10 ug
$3,360.00
PH317773 MAPT MS Standard C13 and N15-labeled recombinant protein (NP_058518) 10 ug
$3,360.00
LC401790 Lyophilized tau (MAPT) 2N4R HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC413819 Lyophilized tau (MAPT) 0N4R HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC413823 Lyophilized tau (MAPT) 0N3R HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426599 Lyophilized tau (MAPT) 1N4R HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401790 Transient overexpression lysate of microtubule-associated protein tau (MAPT) 2N4R (as WB positive control) 100 ug
$665.00
LY413819 Transient overexpression lysate of microtubule-associated protein tau (MAPT) 0N4R (as WB positive control) 100 ug
$436.00
LY413820 Transient overexpression lysate of microtubule-associated protein tau (MAPT), transcript variant 1 (PNS-Tau/Big Tau) (as WB positive control) 100 ug
$665.00
LY413823 Transient overexpression lysate of microtubule-associated protein tau (MAPT) 0N3R (as WB positive control) 100 ug
$436.00
LY426599 Transient overexpression lysate of microtubule-associated protein tau (MAPT) 1N4R (as WB positive control) 100 ug
$436.00
TP313312 Recombinant protein of human microtubule-associated protein tau (MAPT) 2N4R, 20 µg 20 ug
$867.00
TP313364 Recombinant protein of human microtubule-associated protein tau (MAPT) 0N3R, 20 µg 20 ug
$867.00
TP317773 Recombinant protein of human microtubule-associated protein tau (MAPT) 0N4R, 20 µg 20 ug
$867.00
TP720155 Recombinant protein of human microtubule-associated protein tau (MAPT), transcript variant 6 10 ug
$275.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products