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UBE3A (NM_130839) Human Over-expression Lysate

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SKU
LC408892
Lyophilized UBE3A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213039
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_130839, NP_570854
Synonyms ANCR; AS; E6-AP; EPVE6AP; HPVE6A
Predicted MW 100.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_570854
Locus ID 7337
Cytogenetics 15q11.2
Protein Families WB
Protein Pathways Ubiquitin mediated proteolysis
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Other "UBE3A" proteins (7)
SKU Description Size Price
PH313039 UBE3A MS Standard C13 and N15-labeled recombinant protein (NP_570854) 10 ug
$3,360.00
PH316727 UBE3A MS Standard C13 and N15-labeled recombinant protein (NP_000453) 10 ug
$3,360.00
LC400164 Lyophilized UBE3A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400164 Transient overexpression lysate of ubiquitin protein ligase E3A (UBE3A), transcript variant 2 (as WB positive control) 100 ug
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LY408892 Transient overexpression lysate of ubiquitin protein ligase E3A (UBE3A), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP313039 Purified recombinant protein of Homo sapiens ubiquitin protein ligase E3A (UBE3A), transcript variant 3, 20 µg 20 ug
$867.00
TP316727 Recombinant protein of human ubiquitin protein ligase E3A (UBE3A), transcript variant 2, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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