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PPAR gamma (PPARG) (NM_138712) Human Over-expression Lysate

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SKU
LC408559
Lyophilized PPARG HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201538
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_138712, NP_619726
Synonyms CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma
Predicted MW 54.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_619726
Locus ID 5468
Cytogenetics 3p25.2
Protein Families WB
Protein Pathways Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer
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Other "PPAR gamma" proteins (17)
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PH301538 PPARG MS Standard C13 and N15-labeled recombinant protein (NP_619726) 10 ug
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LC402467 Lyophilized PPARG HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC408558 Lyophilized PPARG HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC417568 Lyophilized PPARG HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC430063 Lyophilized PPARG HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY402467 Transient overexpression lysate of peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2 (as WB positive control) 100 ug
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LY408558 Transient overexpression lysate of peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 3 (as WB positive control) 100 ug
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LY408559 Transient overexpression lysate of peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 (as WB positive control) 100 ug
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LY417568 Transient overexpression lysate of peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY430063 Transient overexpression lysate of peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP301538 Recombinant protein of human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1, 20 µg 20 ug
$737.00
TP312181 Recombinant protein of human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 3, 20 µg 20 ug
$737.00
TP312449 Recombinant protein of human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 4, 20 µg 20 ug
$737.00
TP312502 Recombinant protein of human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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