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NOR1 (NR4A3) (NM_173198) Human Over-expression Lysate

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SKU
LC406643
Lyophilized NR4A3 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC223576
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_173198, NP_775290
Synonyms CHN; CSMF; MINOR; NOR1; TEC
Predicted MW 68 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_775290
Locus ID 8013
Cytogenetics 9q31.1
Protein Families WB
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Other "NOR1" proteins (7)
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file-alt Citations

The Orphan Nuclear Receptor Nor1/Nr4a3 Is a Negative Regulator of β-Cell Mass
Close, Dadheech, Villela et al
J. Biol. Chem. (2019) 294 (13), 4889-4897 DOI: 10.1074/jbc.RA118.005135
AI Summary: OriGene products pCMV-Nor1-GFP and pCMV-Nor1-Flag were used in this study for Nor1 overexpression experiments to investigate the role of Nor1 in β-cell apoptosis and mass regulation.
Snippet: loading. Blots were developed on film and band density was determined using ImageJ software. Plasmid Transfection ─ pCMV-Nor1- GFP and pCMV-Nor1-Flag were obtained from Origene (Rockville, MD). pCMV-mCherry
Showing 1-1 of 1 papers.
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