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TCF21 (NM_198392) Human Over-expression Lysate

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SKU
LC405044
Lyophilized TCF21 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC220002
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_198392, NP_938206
Synonyms bHLHa23; POD1
Predicted MW 19.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_938206
Locus ID 6943
Cytogenetics 6q23.2
Protein Families WB
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Other "TCF21" proteins (6)
SKU Description Size Price
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LC418825 Lyophilized TCF21 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY405044 Transient overexpression lysate of transcription factor 21 (TCF21), transcript variant 1 (as WB positive control) 100 ug
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LY418825 Transient overexpression lysate of transcription factor 21 (TCF21), transcript variant 2 (as WB positive control) 100 ug
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TP320002 Recombinant protein of human transcription factor 21 (TCF21), transcript variant 1, 20 µg 20 ug
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TP761400 Purified recombinant protein of Human transcription factor 21 (TCF21), transcript variant 2, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap
Nurnberg, Cheng, Raiesdana et al
PLoS Genet. (2015) 11 (5), e1005155 DOI: 10.1371/journal.pgen.1005155
Cited by 105
AI Summary: In this study, OriGene's siRNA pools targeting TCF21 and control siRNA (siTCF21; siCONTROL) were used to knock down TCF21 expression in human coronary artery smooth muscle cells, enabling the authors to investigate TCF21's role in regulating genes and pathways relevant to coronary artery disease.
Snippet: Experimental factors, siRNA treatment: siTCF21;siCONTROL. Experimental features, HCASMCs were purchased from Lonza and cultured according to the manufacturer's recommendations. Cells were serum-starved and transfected with siRNA pools from OriGene ... Cite SaveSaving
Showing 1-1 of 1 papers.
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