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NOTCH1 (NM_017617) Human Over-expression Lysate

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SKU
LC402602
Lyophilized NOTCH1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211365
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_017617, NP_060087
Synonyms AOS5; AOVD1; hN1; TAN1
Predicted MW 272.58 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
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Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_060087
Locus ID 4851
Cytogenetics 9q34.3
Protein Families WB
Protein Pathways Dorso-ventral axis formation, Notch signaling pathway, Prion diseases
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Other "NOTCH1" proteins (3)
SKU Description Size Price
LY402602 Transient overexpression lysate of Notch homolog 1, translocation-associated (Drosophila) (NOTCH1) (as WB positive control) 100 ug
$665.00
TP750178 Purified recombinant protein of Human notch 1 (NOTCH1), Glu1448-Gln1734, tag free, expressed in E.Coli, 50 ug 50 ug
$270.00
TP762041 Purified recombinant protein of Human notch 1 (NOTCH1),Ala2280-End, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Notch signaling plays a crucial role in cancer stem-like cells maintaining stemness and mediating chemotaxis in renal cell carcinoma
Xiao, Gao, Duan et al
J. Exp. Clin. Cancer Res. (2017) 36 (1), 41 DOI: 10.1186/s13046-017-0507-3
AI Summary: OriGene products pCMV6-AC-GFP-Numb and pCMV6-AC-GFP-Notch1 were used in this study to modulate Notch signaling in renal cell carcinoma stem-like cells, enabling the investigation of Notch pathway effects on cancer cell stemness and chemotaxis.
Snippet: The parental cells, CD133 + /CD24 + sorted cells or CD133 + /CD24 + sorted cells transfected with its endogenous inhibitor Numb vector pCMV6-AC-GFP-Numb (ORIGENE) or notch 1 NICD overexpression VectorpCMV6-AC-GFP-Notch1 (ORIGENE) using lipofectin2000 ... All 8 versions Cite SaveSaving
FigureFigureFigure
MiR-34c inhibits osteosarcoma metastasis and chemoresistance
Xu, Jin, Xu et al
Med. Oncol. (2014) 31 (6), 972 DOI: 10.1007/s12032-014-0972-x
Cited by 55
AI Summary: OriGene’s Notch1 plasmid was used in this study to investigate the role of Notch1 as a target gene of miR-34c, helping to elucidate how miR-34c suppresses osteosarcoma metastasis and chemoresistance.
Snippet: All reagents for RNA isolation and real-time PCR were purchased from Life Technologies. Cell viability and invasion assays The indicated cells were transfected with the indicated nucleotide and/or a Notch1 plasmid (Origene, Rockvill, MD, USA). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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