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MDM2 (NM_002392) Human Over-expression Lysate

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SKU
LC400855
Lyophilized MDM2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219518
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002392, NP_002383
Synonyms ACTFS; hdm2; HDMX; LSKB
Predicted MW 55.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002383
Locus ID 4193
Cytogenetics 12q15
Protein Families WB
Protein Pathways Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis
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Other "MDM2" proteins (2)
SKU Description Size Price
LY400855 Transient overexpression lysate of Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2 (as WB positive control) 100 ug
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TP761916 Purified recombinant protein of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, Gln119-Leu438, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells
Pal, Mukhopadhyay, Ramaiah et al
PLoS ONE (2016) 11 (7), e0159092 DOI: 10.1371/journal.pone.0159092
Cited by 25
AI Summary: In this study, the OriGene product Mdm2 antibody was used to investigate the molecular mechanisms by which miR-203 regulates cell proliferation and migration in human glioblastoma cells.
Snippet: anti cytochrome C (1:1000) (purchased from Abbiotech), anti E-cadherin (1:1000), anti MMP-9 (1:1000), anti Snail1 (1:1000),anti p53(1:1000) and anti caspase-3 (1:1000) (purchased from Cell Signaling Technology), Mdm2 (1:1000) (purchased from Origene), N- ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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