MDM2 (NM_002392) Human Tagged ORF Clone
SKU
RC219518
MDM2 (Myc-DDK-tagged)-Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MDM2 |
| Synonyms | ACTFS; hdm2; HDMX; LSKB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002392 |
| ORF Size | 1491 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002392.5 |
| RefSeq Size | 2357 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 4193 |
| UniProt ID | Q00987 |
| Cytogenetics | 12q15 |
| Domains | MDM2, zf-RanBP |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis |
| MW | 55.8 kDa |
| Summary | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. provided by RefSeq, Jun 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002392" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC219518L1 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC219518L2 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, mGFP tagged | 10 ug |
$986.00
|
|
| RC219518L3 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC219518L4 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, mGFP tagged | 10 ug |
$986.00
|
|
| RG219518 | MDM2 (tGFP-tagged) - Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2 | 10 ug |
$886.00
|
|
| SC118660 | MDM2 (untagged)-Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2 | 10 ug |
$686.00
|
|
Citations
The ubiquitin ligase MDM2 sustains STAT5 stability to control T cell-mediated antitumor immunity
Zhou, Kryczek, Li et al
Nat Immunol (2021) 22 (4), 460-470 DOI: 10.1038/s41590-021-00888-3
Zhou, Kryczek, Li et al
Nat Immunol (2021) 22 (4), 460-470 DOI: 10.1038/s41590-021-00888-3
Cited by 108
AI Summary: OriGene products Myc–DDK–MDM2 (RC219518) and Myc–DDK–STAT5 (RC205753) were used in this study to investigate the molecular interactions and stability of MDM2 and STAT5 in T cells, helping to elucidate their roles in regulating antitumor immunity.
Snippet: Targeting the p53-MDM2 pathway to reactivate tumor p53 is a chemotherapeutic approach. However, the involvement of this pathway in CD8+ T cell-mediated antitumor immunity is unknown. Here, we report that mice with MDM2 deficiency in T cells exhibit accelerated;Myc–DDK–MDM2 (RC219518) and Myc–DDK–STAT5 (RC205753) were from OriGene Technologies
A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis
Zhang, Cao, Gong et al
Theranostics (2020) 10 (23), 10769-10790 DOI: 10.7150/thno.46878
Zhang, Cao, Gong et al
Theranostics (2020) 10 (23), 10769-10790 DOI: 10.7150/thno.46878
Cited by 18
AI Summary: In this study, the OriGene product pCMV6-MDM2 was used as the template to amplify FLAG-tagged MDM2, enabling the investigation of MDM2’s role in the ECT2/USP7 regulatory circuit in breast carcinogenesis.
Snippet: mutation assay. FLAG tagged ECT2 truncations were generated by PCR cloning and carried by pLenti vector. The FLAG tagged MDM2 was amplified from pCMV6-MDM2 (Origene) and carried by pLenti-hygro vector. The Rluc
High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq)
Andrews, Schaefer-Ramadan, Al-Thani et al
J Biol Chem (2019) 294 (30), 11549-11558 DOI: 10.1074/jbc.RA119.008792
Andrews, Schaefer-Ramadan, Al-Thani et al
J Biol Chem (2019) 294 (30), 11549-11558 DOI: 10.1074/jbc.RA119.008792
AI Summary: OriGene contributed to this study by providing six human gene clones (PKAR2 [RC220376], AKAP5 [RC221314], MDM2 [RC219518], p53 [RC200003], FOS [RC202597], and JUN [RC209804] in pCMV6-Entry vectors), which were used as source material for protein-protein interaction mapping in the AVA-Seq system.
Snippet: … Despite their success;The following six human genes were purchased from Origene as 10-μg stocks in pCMV6-Entry vector with SgfI and MluI cloning sites: PKAR2 (RC220376;1212 bp), AKAP5 (RC221314;1281 bp), MDM2 (RC219518;1491 bp), p53 (RC200003;1179 bp), FOS (RC202597;1140 bp), and JUN (RC209804;993 bp). …
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
