MDM2 (NM_002392) Human Untagged Clone
SKU
SC118660
MDM2 (untagged)-Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | MDM2 |
| Synonyms | ACTFS; hdm2; HDMX; LSKB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_002392 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002392.2, NP_002383.2 |
| RefSeq Size | 2357 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 4193 |
| UniProt ID | Q00987 |
| Cytogenetics | 12q15 |
| Domains | MDM2, zf-RanBP |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis |
| Summary | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. provided by RefSeq, Jun 2013 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002392" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC219518 | MDM2 (Myc-DDK-tagged)-Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2 | 10 ug |
$686.00
|
|
| RC219518L1 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC219518L2 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, mGFP tagged | 10 ug |
$986.00
|
|
| RC219518L3 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC219518L4 | Lenti ORF clone of Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2, mGFP tagged | 10 ug |
$986.00
|
|
| RG219518 | MDM2 (tGFP-tagged) - Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2 | 10 ug |
$886.00
|
|
Citations
Corosolic acid inhibits cancer progress through inactivating YAP in hepatocellular carcinoma
Jia, Xiong, Li et al
Oncol. Res. (2020)
Jia, Xiong, Li et al
Oncol. Res. (2020)
AI Summary: OriGene products, including the YAP overexpressing lentivirus vector (Vector-YAP) and the short hairpin RNA targeting MDM2 (sh-MDM2), were used in this study to manipulate gene expression and investigate the molecular mechanisms by which corosolic acid inhibits cancer progression in hepatocellular carcinoma.
Snippet: … The short hairpin RNAs (shRNAs) used to downregulate human MDM2 (sh-MDM2) and;- control vector (Vector-NC), as well as the overexpressing lentivirus vector used to upregulate YAP (Vector-YAP) were all purchased from OriGene (Rockville, MD, USA). The short hairpin RNAs (shRNAs) used to downregulate human MDM2 (sh-MDM2) and
Nandrolone, an anabolic steroid, stabilizes Numb protein through inhibition of mdm2 in C2C12 myoblasts
Liu, Yao, Levine et al
J. Androl. (2012) 33 (6), 1216-23 DOI: 10.2164/jandrol.112.016428
Liu, Yao, Levine et al
J. Androl. (2012) 33 (6), 1216-23 DOI: 10.2164/jandrol.112.016428
Cited by 13
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
