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IDH2 (NM_002168) Human Over-expression Lysate

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SKU
LC400787
Lyophilized IDH2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201152
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002168, NP_002159
Synonyms D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Predicted MW 50.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002159
Locus ID 3418
Cytogenetics 15q26.1
Protein Families WB
Protein Pathways Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways
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TP710216 Purified recombinant protein of mutant(R172K) of Homo sapiens isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2),with C-terminal DDK tag,expressed in sf9 cells, 20 µg 20 ug
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TP710217 Purified recombinant protein of Homo sapiens isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2), nuclear gene encoding mitochondrial protein, full length, with C-terminal HIS tag, expressed in sf9, 20ug 20 ug
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TP710228 Purified recombinant protein of Homo sapiens isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2) and mutant(R172G) heterodimer, with C-terminal 6xHis and DDK tags, expressed in sf9 cells, 20 µg 20 ug
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TP710229 Purified recombinant protein of Homo sapiens isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2) and mutant(R172M) heterodimer, with C-terminal 6xHis and DDK tags, expressed in sf9 cells, 20 µg 20 ug
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TP710230 Purified recombinant protein of Homo sapiens isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2) and mutant(R172K) heterodimer, with C-terminal 6xHis and DDK tags, expressed in sf9 cells, 20 µg 20 ug
$540.00

file-alt Citations

On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel
Heo, Kim, Lee et al
PLoS ONE (2016) 11 (1), e0147794 DOI: 10.1371/journal.pone.0147794
Cited by 12
AI Summary: OriGene contributed human IDH1 (Cat# NM_005896) and IDH2 (Cat# NM_002168) gene products, which were used for PCR amplification and subsequent cloning into lentiviral vectors to create IDH1/2 mutant-overexpressing glioma models in this study.
Snippet: Specifically, the genes for human IDH1 (GenBank accession number NM_005896) and IDH2 (GenBank accession number NM_002168) were obtained from Origene (Rockville, MD, USA), PCR amplified, and cloned into lentiviral vector CD526A–1 (System Biosciences ... Cite SaveSaving
FigureFigureFigure
Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
Wen, Cho, Yun et al
J. Neurochem. (2014)
Cited by 17
AI Summary: OriGene contributed to this study by providing human IDH1 (NM_005896) and IDH2 (NM_002168) gene products, which were used to generate wild type and mutant constructs for transfection and subsequent metabolomic analysis.
Snippet: Lentiviral vector construction and preparation The genes for human IDH1 (GenBank accession number NM_005896) and IDH2 (GenBank accession number NM_002168) were obtained from Origene (Rockville, MD, USA), PCR-amplified and cloned into ... Cite SaveSaving
Showing 1-2 of 2 papers.
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