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IGF1 Receptor (IGF1R) (NM_000875) Human Over-expression Lysate

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SKU
LC400311
Lyophilized IGF1R HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214928
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000875, NP_000866
Synonyms CD221; IGFIR; IGFR; JTK13
Predicted MW 154.79 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000866
Locus ID 3480
Cytogenetics 15q26.3
Protein Families WB
Protein Pathways Adherens junction, Colorectal cancer, Endocytosis, Focal adhesion, Glioma, Long-term depression, Melanoma, Oocyte meiosis, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer
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Other "IGF1 Receptor" proteins (5)
SKU Description Size Price
PH314928 IGF1R MS Standard C13 and N15-labeled recombinant protein (NP_000866) 10 ug
$3,360.00
LY400311 Transient overexpression lysate of insulin-like growth factor 1 receptor (IGF1R) (as WB positive control) 100 ug
$665.00
TP314928 Recombinant protein of human insulin-like growth factor 1 receptor (IGF1R), 20 µg 20 ug
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TP700109 Purified recombinant protein of Human insulin-like growth factor 1 receptor (IGF1R), with C-terminal DDK/His tag, expressed in human cells 20 ug
$900.00
TP710101 Recombinant protein of human insulin-like growth factor 1 receptor (IGF1R), residues 741-935aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00

file-alt Citations

miR-195 inhibits the growth and metastasis of NSCLC cells by targeting IGF1R
Wang, Wang, Lan et al
Tumour Biol. (2014)
Cited by 43
AI Summary: OriGene contributed to this paper by providing the human IGF1R coding sequence (NM_000875), which was used to study the effects of IGF1R restoration on miR-195-mediated suppression of NSCLC cell growth and metastasis.
Snippet: Plasmids Mimics for miR-195 and control (miR-NC) were obtained from Ribobio (Guangzhou). The coding sequence of human IGF1R (NM_000875) was purchased from Origene (Rock- ville, MD, USA) and cloned into pcDNA4. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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