Prph Mouse Gene Knockout Kit (CRISPR)

CAT#: KN313978

Prph - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Prph
• Locus ID: 19132
• Components:

  KN313978G1, Prph gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTGATGATGGCTCATGCTGG

  KN313978G2, Prph gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGAGTAGGAGAAAGCCCCGG

  KN313978D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CTTTACCGTG CCCTGGGGAG TAGGCAGAGA TAAGCTCTTC CCCAGCTCCC TCTGCCTCAG CCCTCGGTTG TGGCCAATGA TGGGGGGCAG TTGACAACAG GTGAAAGGAG AACCCCAGTT TCAGGAGACA GGAGGAGGCA CGAATTCCCT GGCTTAGGCC AGGTTAGCTC TCCCTCCACC TACCCCACTT CTCATTGCTC AAAACTTGCC CTTTTCCTCA GGTCCTCATA TTCCCTAATT TTTACCCCCT CTTCTGAGAG GGCACCCCAG GTCAAGCCAT GTCCTCCCAT TCTAGGCTCC AGCGTTGGAT GCATGCTCTA AGGTAGACCT TAGCCCACCT CCATCACATC CCGGATCTCA GCCAGCAACA AGGGGGAATC AAGCAGGCAG GGTGCCAGCA ACCAGGAGAG GGAAGGGGTG GTGTCCTCTC TCTGCAGGGT GGGGCATCCC CCTCCCCACA CAGCCCAAGG CTGAAGTCAG GCCAGTGGGA GGAGCTGTCG TGGCCCCCCA CCCCCCCTCC CCGGAGACCG CAGGGCTATA AAGCCGCCCC GCATCGGTCT GCAGCTCCTT GCCACCCGGC CTAGTTCTGC CAAGCGCTGA TCCAGCAGCC GCCTGCTGGG CTCGGGGTCC CCGAGCTCCT CGGCGCGGCT GGGCAGCTTC CGTGCCCCTC GAGCGGGGGC ACTGCGCTTG CCCTCGGAGC GCCTCGATTT CTCCATGGCC GAGGCCCTCA ACCAAGAGTT CCTGGCCACT CGGAGCAACG AGAAGCAAGA GTTACAGGAG CTCAACGACC GCTTCGCCAA CTTCATCGAG AAGGTGCGCT TCTTGGAGCA GCAGAACGCA GCCCTGCGCG GGGAGCTGAG CCAGGCGCGG GGCCAGGAGC CGGCGCGCGC CGACCAGCTT TGCCAGCAGG AGCTGCGCGA GCTGCGGCGC GAACTGGAGC TGCTGGGCCG GGAGCGCGAC CGGGTGCAGG TGGAGCGGGA CGGGCTGGCG GAGGACCTAG CGGCGCTCAA GCAGAGGTCA GAGGGCAGGA CCGGGCCTGC CAGACGCTCC CTTCGCTCCC CCTGCCTTCC CCTCCAGATC CAACAGCCCT AGGGCGTGGC CGGGCTGGCC AACCCCAGGG CGAGCCTGCA GCATCCAAGC CAGCCCCCTC CCTGCACTTT GCTCCGAGAG CCTAGCGGAA GTGACCAGAC ATTAAGGGGA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001163588, NM_001163589, NM_013639
• Synonyms: Prph1