Prph (NM_001163589) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Prph |
| Synonyms | Prph1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001163589 |
| ORF Size | 1425 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001163589.1, NP_001157061.1 |
| RefSeq Size | 1769 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 19132 |
| Cytogenetics | 15 55.72 cM |
| MW | 54.3 kDa |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001163589" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC218098 | Prph (untagged) - Mouse peripherin (Prph), transcript variant 3, (10ug) | 10 ug |
$503.00
|
|
| MR207614L3 | Lenti ORF clone of Prph (Myc-DDK-tagged) - Mouse peripherin (Prph), transcript variant 3 | 10 ug |
$757.00
|
|
| MR207614L4 | Lenti ORF clone of Prph (mGFP-tagged) - Mouse peripherin (Prph), transcript variant 3 | 10 ug |
$757.00
|
|
Citations
Enterovirus-A71 exploits peripherin and Rac1 to invade the central nervous system
Lim, Ng, Oo et al
EMBO Rep (2021)
Lim, Ng, Oo et al
EMBO Rep (2021)
Cited by 23
AI Summary: OriGene’s pCV6-entry-PRPH plasmid was used in this study to express peripherin and investigate its role in facilitating EV-A71 entry and replication in neural cells, thereby elucidating a unique mechanism of viral neuroinvasion.
Snippet: … Article Enterovirus-A71 exploits peripherin and Rac1 to invade the central nervous system Ze Qin Lim 1,2 , Qing Yong Ng 1,2 , Yukei Oo 1,2 , Justin Jang Hann Chu 1 , Shi Yan Ng 3 , Siu Kwan Sze 4 & Sylvie Alonso 1,2,* Abstract;E. coli harboring LucEV‐A71 replicon (Wu & Chu, 2017) and pCV6‐entry‐PRPH (OriGene) were grown in LB broth supplemented with Kanamycin (50 μg/ml). …
Showing 1-1 of 1 papers.
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