Notch3 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN311126RB

Notch3 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Notch3
• Locus ID: 18131
• Components:

  KN311126G1, Notch3 gRNA vector 1 in pCas-Guide CRISPR vector

  KN311126G2, Notch3 gRNA vector 2 in pCas-Guide CRISPR vector

  KN311126RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_008716
• UniProt ID: Q61982
• Synonyms: AW229011; hpbk; N3
• Summary: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play a role during CNS development.[UniProtKB/Swiss-Prot Function]