Ctsb Mouse Gene Knockout Kit (CRISPR)
$1,657.00
4 Weeks*
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
|---|---|
| Vector | GFP-puro |
| Target Symbol | Ctsb |
| Locus ID | 13030 |
| Components |
KN303984G1, Ctsb gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGGCACTGGTCAGTGCCAGC KN303984G2, Ctsb gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTAATCAGGTCATCCGACAG KN303984D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences: pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACGTCAC ACGTACAGGC GAACAGAAGG AATTTAACAT GGCCCTTTAC GTGTCTTTAA GTGAGGGACT TGTAAACCAG GCTTTTTAGT TTCCTTATGG ATTGACTCTC CTTAGGACTC ACACTGTTTG ATGGTTTTGG GTATTTTTGT TTTGTTTTTT AAATTTAATG ACTTTTATTT TTTCACTTAT TTACTTTACA TCTGGCTCAC TGTTCCCCCT CCCAGTCACA CCCCCTCCCC CCATACACAC ATATAATCTT TTTTTGTGTT GTTTTGTTTT GTTGAGGCAG GCTGTAGCTA CATGGCCCAG GCTGAACTCT GTCTTAATAC CTTAGAATTC CCAGTACAGA GTTTATACTA CATCCACCTT AACGCTGACT CTTCTTCTAG AACACTGGAG AGAATCTCTT AAACTTAGTT CATCAATTTA AAAATAGCCA GGAGGGGACA TACATATATT GTTGTGTGTG ATATCTGCTC CCATCCAACC AGGCTTCACA GTGAGGCCAG AAAGGCTGCA AGAGGCCCGA GTCATGCCCG TGTGTAGAAA TCATTTTGAT GTTGCCTGTG TCCTTTCTAG GTGAGTGCAG GATCCAGCAT CCAGGACTAG CATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAGAAT TCCGATCATA TTCAATAACC CTTAATATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT TAGGTCTGAA GAGGAGTTTA CGTCCAGCCA AGCTTAGGAT CTCGACCTCG AAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGAC TCCGTTCTTT GGTGGCCCCT TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCGCCCCG CAGCTCGCGT CGTGCAGGAC GTGACAAATG GAAGTAGCAC GTCTCACTAG TCTCGTGCAG ATGGACAGCA CCGCTGAGCA ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG CCAATAGCAG CTTTGCTCCT TCGCTTTCTG GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG GGGCGGGCTC AGGGGCGGGC TCAGGGGCGG GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC GGCATTCTGC ACGCTTCAAA AGCGCACGTC TGCCGCGCTG TTCTCCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCATCC ATCTAGATCT CGAGCAGCTG AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCACAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT GCATCGATGA TATCAGATCC CCGGGATGCA GAAATTGATG ATCTATTAAA CAATAAAGAT GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA GAAGGGTGAG AACAGAGTAC CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG GTGGGGTGGG ATTAGATAAA TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG ATAATGTTTC ATAGTTGGAT ATCATAATTT AAACAAGCAA AACCAAATTA AGGGCCAGCT CATTCCTCCC ACTCATGATC TATAGATCTA TAGATCTCTC GTGGGATCAT TGTTTTTCTC TTGATTCCCA CTTTGTGGTT CTAAGTACTG TGGTTTCCAA ATGTGTCAGT TTCATAGCCT GAAGAACGAG ATCAGCAGCC TCTGTTCCAC ATACACTTCA TTCTCAGTAT TGTTTTGCCA AGTTCTAATT CCATCAGAAG CTGGTCGAGA TCCGGAACCC TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTCCCTCG AAGAGGTTCA CTAGGCGCGC CTTCCGGTCT GTCTGTGTGT CTGTCTTTGC AGGTCCCTTG TGCACACTGC CTCTGAGGTG ACTAACGGGC TGTAAGGCAT TCTTTTCTGC AAGTTGAAGC TCATAGACCA CACCATGGAT TGCTCTCCAG TCTCCATGTT GTGTGCATGG GAGTGCACAC ACATGTGAAT CCTTGTACAT GGAGGCGTAT GGGGGGGATA AGTGAGCTTT GGAGCGAGTT GGACCCTGGG AAAACTCCTA TGGGAGATGA GGAAATTCCA AACTACTTCC TGCCGTCATT TCCTCCAGAT CTGGGGCAGG TGTCCAGCTA ACAACTGAAA GGAGATTAGA GAAGACACTG TGGTCTGAGA GCTGAGCTCT GACCCTGAAT CCCTCTGCCT GTTCCCTAGC TCCCTGAATT GGACAGAACC CCTGATCCAC TCATCCCATC ATGGGAAACC CTGTGGTAGG TAGGTGACTG TCCTGGTGTG ACTCTCTTTC AGGCTGGACG CAACTTCTAC AATGTTGACA TAAGCTATCT GAAGAAGCTG TGTGGCACTG TCCTGGGTGG ACCCAAACTG CCAGGAAGGT GAGTGACACA GCCTGGAGTG GACACTGCCC CTCACTCTCG CCGGTTGGAC TTTAGATCAG AAGGGATCTT GCTGCCGCCC GAAAGAGGAA GGGCTGGAAG AGGAAGGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCGE100003, scramble sequence in pCas-Guide vector |
| OTI Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_007798 |
| UniProt ID | P10605 |
| Synonyms | CB |
| Summary | This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. provided by RefSeq, Aug 2015 |
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| KN303984LP | Ctsb - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit |
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| KN303984RB | Ctsb - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit |
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| KN503984 | Ctsb - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 kit |
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Citations
Compartment-specific inhibition of cytosolic Cathepsin B mitigates microglial senescence in the aging brain
Zhang, Zhang, Mao et al
Pharmacol Res (2026) 233, 108478 DOI: 10.1016/j.phrs.2026.108478
Zhang, Zhang, Mao et al
Pharmacol Res (2026) 233, 108478 DOI: 10.1016/j.phrs.2026.108478
AI Summary: OriGene's Ctsb Mouse Gene Knockout Kit (CRISPR) was used to generate Cathepsin B-deficient microglial cells, enabling the study of cytosolic Cathepsin B’s role in microglial senescence in this paper.
Cathepsin B deficiency disrupts cortical development via PEG3, leading to depression-like behavior
Xie, Yang, Lan et al
Commun Biol (2025) 8 (1), 1097 DOI: 10.1038/s42003-025-08508-8
Xie, Yang, Lan et al
Commun Biol (2025) 8 (1), 1097 DOI: 10.1038/s42003-025-08508-8
Cited by 4
AI Summary: OriGene’s scramble control gRNA (SKU GE100003, Cat# KN303984) was used as a negative control in gene knockout experiments to validate the effects of Cathepsin B deficiency on neuronal development in this study.
Snippet: During the generation, we also included scramble control gRNA (SKU GE100003, ORIGENE, Cat#KN303984) to serve as a negative control. …CTSB expression was assessed by Q-PCR and immunoblotting. During the generation, we also included scramble control gRNA (SKU GE100003, ORIGENE, Cat#KN303984) to serve as a negative control. …
Cathepsin B Modulates Alzheimer's Disease Pathology Through SAPK/JNK Signals Following Administration of Porphyromonas gingivalis-Derived Outer Membrane Vesicles
Jiang, Ge, Yin et al
J Clin Periodontol (2024)
Jiang, Ge, Yin et al
J Clin Periodontol (2024)
Cited by 13
Cathepsin B modulates microglial migration and phagocytosis of amyloid β in Alzheimer's disease through PI3K-Akt signaling
Jiang, Zhao, Zhou et al
Neuropsychopharmacology (2024)
Jiang, Zhao, Zhou et al
Neuropsychopharmacology (2024)
Cited by 17
Cathepsin B inhibition blocks neurite outgrowth in cultured neurons by regulating lysosomal trafficking and remodeling
Jiang, Meng, Zeng et al
J. Neurochem. (2020)
Jiang, Meng, Zeng et al
J. Neurochem. (2020)
Cited by 28
AI Summary: OriGene’s CRISPR and homology-directed repair kit (Cat# KN303984) was used to generate CTSB knockout N2a neuronal cells, enabling the study to demonstrate the essential role of cathepsin B in neurite outgrowth and lysosomal trafficking.
Snippet: commercially available clustered regularly interspaced short palindromic repeats (CRISPR) and homology-directed repair (HDR) (ORIGENE, Cat#KN303984) on N2a cells via CRISPR and HDR ORIGENE, Cat#KN303984) to serve as a negative control;- commercially available clustered regularly interspaced short palindromic repeats (CRISPR) and homology-directed repair (HDR) (ORIGENE, Cat#KN303984) on N2a cells via CRISPR and HDR ORIGENE, Cat#KN303984) to serve as a negative control
Showing 1-5 of 5 papers.
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