C5ar1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN302383RB

C5ar1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: C5ar1
• Locus ID: 12273
• Components:

  KN302383G1, C5ar1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN302383G2, C5ar1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN302383RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001173550, NM_007577
• UniProt ID: P30993
• Synonyms: C5aR; C5r1; Cd88; D7Msu1
• Summary: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.[UniProtKB/Swiss-Prot Function]