C5ar1 (NM_007577) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR225064
C5ar1 (Myc-DDK-tagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | C5ar1 |
| Synonyms | C5aR; C5r1; Cd88; D7Msu1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007577 |
| ORF Size | 1053 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007577.1, NM_007577.2, NM_007577.3, NM_007577.4, NP_031603.2 |
| RefSeq Size | 2481 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 12273 |
| UniProt ID | P30993 |
| Cytogenetics | 7 8.77 cM |
| MW | 39.5 kDa |
| Summary | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007577" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208206 | C5ar1 (untagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1, (10ug) | 10 ug |
$686.00
|
|
| MG225064 | C5ar1 (tGFP-tagged) - Mouse complement component 5a receptor 1 (C5ar1) transcript variant 1, (10ug) | 10 ug |
$886.00
|
|
| MR225064L3 | Lenti ORF clone of C5ar1 (Myc-DDK-tagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1 | 10 ug |
$986.00
|
|
| MR225064L4 | Lenti ORF clone of C5ar1 (mGFP-tagged) - Mouse complement component 5a receptor 1 (C5ar1), transcript variant 1 | 10 ug |
$986.00
|
|
Citations
MK801 regulates the expression of key osteoarthritis factors in osteoarthritis synovial fibroblasts through complement C5
Huang, Feng, Zhu et al
Res Vet Sci (2021) 136, 377-384 DOI: 10.1016/j.rvsc.2021.03.003
Huang, Feng, Zhu et al
Res Vet Sci (2021) 136, 377-384 DOI: 10.1016/j.rvsc.2021.03.003
Cited by 2
AI Summary: In this study, OriGene's mouse C5 siRNA (SR420963) and mouse C5 overexpression plasmid (MR226017) were used to modulate complement C5 expression in osteoarthritis synovial fibroblasts, enabling the authors to demonstrate the role of C5 in mediating MK801’s effects on inflammatory and osteoarthritis-related factors.
MK801 regulates the expression of key osteoarthritis factors in osteoarthritis synovial fibroblasts through complement C5
Huang, Feng, Zhu et al
Research in Veterinary Science (2021) 136, 377-384 DOI: 10.1016/j.rvsc.2021.03.003
Huang, Feng, Zhu et al
Research in Veterinary Science (2021) 136, 377-384 DOI: 10.1016/j.rvsc.2021.03.003
Cited by 2
AI Summary: OriGene products, specifically the C5 shRNA vector (CAT#: TL511576) and C5 overexpression vector (CAT#: MR225064), were used to manipulate complement C5 expression in osteoarthritis synovial fibroblasts, enabling the study of its role in the regulation of osteoarthritis marker factors by MK801.
Primary squamous cell carcinoma of the extrahepatic bile duct: A case report
Delgado Maroto, Barrientos Delgado, Lázaro Sáez et al
Gastroenterol Hepatol (2019) 42 (7), 436-437 DOI: 10.1016/j.gastrohep.2019.03.003
Delgado Maroto, Barrientos Delgado, Lázaro Sáez et al
Gastroenterol Hepatol (2019) 42 (7), 436-437 DOI: 10.1016/j.gastrohep.2019.03.003
AI Summary: OriGene Technologies contributed to the study by providing the pCMV6 mouse C5aR1 plasmid and mouse CD177 cDNA, which were used to construct and characterize CHO cell lines expressing these proteins.
Snippet: … Suvorova et al. (2005) previously described the construction and characterization of CHO cells that express human or mouse CD177 and C5aR1 or C5aR1-GFP. Human CD177 cDNA in the pDNA-Dual plasmid can be purchased from the DNASU plasmid repository. Mouse CD177 cDNA and pCMV6 mouse C5aR1 can be purchased from OriGene Technologies. …
Showing 1-3 of 3 papers.
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