Atg5 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN301740BN

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Atg5 - mouse gene knockout kit via CRISPR, HDR mediated

HDR-mediated knockout kit validation

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USD 1,290.00

4 Weeks

    • 1 kit

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Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA mBFP-Neo
Symbol Atg5
Locus ID 11793
Kit Components

KN301740G1, Atg5 gRNA vector 1 in pCas-Guide CRISPR vector

KN301740G2, Atg5 gRNA vector 2 in pCas-Guide CRISPR vector

KN301740BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001314013, NM_053069, NM_001358596
Synonyms 2010107M05Rik; 3110067M24Rik; Apg5l; Atg5l; AW319544; C88337; Paddy
Summary The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Two transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]


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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
68 Mouse Clones
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