LRIG1 Human Gene Knockout Kit (CRISPR)

CAT#: KN223584RB

LRIG1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: LRIG1
• Locus ID: 26018
• Components:

  KN223584G1, LRIG1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCGGCCGCCTGCACTTGCGC

  KN223584G2, LRIG1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTGGCTGCTTTTGCTTCGGC

  KN223584RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  CCGCGCCGTC CAGCCCGAGG TCTACGGCTT TGCGCTCCGA GCCCAGAGGA AGATGCCTGC CGGCACCGAG CTCGGGCTGC GGGGCTGAAC GCCTGTCTTC CAGGCGCAGC GGCAGCACTG CCCTCGGCCG GTGTCGGTAG CGGCACTCGG CGTGCCCCGG GCGGACGAAG AGCGCAGGCT GGGTACACCT TGCCCGAATC GGCGGAGTTC GCAGCTAGCG CGGGCGGGCC GGCCGGCCCG GATGGGCGCG GGGTTTGCGG CCCCCGCCGG GTGCTCCGGA GCGGCCCGGG CACCGGGGGC ACGCTGAGTG CCGGAGCCGC GGCCGCAGAG AGAACTTGGG GCGGGGGCCA TGCCCCGGTG CGGAGTCTAG AGCCGAGCGG AGCGCCCCGC GGGCCCGCCC GCGTCTGCGG CCGTTCGGGT CCTCACAGTC CCCGCGCCCG GAGTTCGCAG CGCGCTCCAG ACAAGACTCG CTGGACTGCG GTGGGCGCGG GCTGGCTGCG TTGCCCGGGG ACCTGCCCTC CTGGACGCGG AGCCTGTGAG TGCCGCCCTT TCCGCCCTCT CTGCGCCCCG GGGCTCTGTC ACTTGGGGAT GGAGCGGCGG GGGTTCGTAC TCAGGGAGGG GGTGCGGACT GGGTTGTGTG TGTGGAGTTG TGCGTGCTTG TGCGTGTTGG AAGACTGTCA GGTTGGAGTG GCGGAAGGGA GATTCTGCGT GGAGGTCCGG GGGAGTAGGC GTGCGAGAGA GCCTGGAGAA GAAGAGCGTG CCTCGTGGGG TCTGGAGACG AGCGAGCGTG GGTCGTAGAG ATTTGGGGGT TAGCCAGCAC GCGTCGTGCG GGCCATCTGG GGGTGAGTGA GTGGGAGTCT GGAGCTGAGT GTGTTTGCGG GTGAGTGGGT GGGAAACGTC TGGAGAAGAG TGTGAAGAAG TCTGTGGGGG TATGTGGGTG CGTTTCCTTT TCCTCTCCTG TAATCCAACT ATTTGGTGTC TGAGCTCCTT GGTCTTCCAG TGGGTAATGA ATGAAGGTGA CTGTTGGGTC CAGTCTTTCG TTTACATTAT TTATTAGTTG ATAGT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_015541
• UniProt ID: Q96JA1
• Synonyms: LIG-1; LIG1
• Summary: Acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.[UniProtKB/Swiss-Prot Function]