DNMT3B Human Gene Knockout Kit (CRISPR)

CAT#: KN221966RB

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DNMT3B - human gene knockout kit via CRISPR, HDR mediated



HDR-mediated knockout kit validation


USD 1,657.00


Availability*
4 Weeks

Size
    • 1 kit

Product images

Specifications

Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA RFP-BSD
Symbol DNMT3B
Locus ID 1789
Kit Components

KN221966G1, DNMT3B gRNA vector 1 in pCas-Guide CRISPR vector

KN221966G2, DNMT3B gRNA vector 2 in pCas-Guide CRISPR vector

KN221966RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001207055, NM_001207056, NM_006892, NM_175848, NM_175849, NM_175850
UniProt ID Q9UBC3
Synonyms ICF; ICF1; M.HsaIIIB
Summary CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.