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NF-kB p65 (RELA) Human Gene Knockout Kit (CRISPR)

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SKU
KN220780RB
RELA - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Vector RFP-BSD
Target Symbol NF-kB p65
Locus ID 5970
Components

KN220780G1, NF-kB p65 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCCGCGGGGTGGCATCGCCG

KN220780G2, NF-kB p65 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGGGCCGCCGCGGCTGTGGC

KN220780RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

Homologous arm and RFP-BSD sequences:

pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

CCGCATCTAG ATTGGGGTGG GTAGGACATT TTAACGTGCT TCTGAGACAG CCACCACCGA AAGGCACCTT TAGCGGTTAG GAAACCCGCG CGGAGTTCAA CCACCCGGCC TCTGCGGAGC AGAAGGCCCG GTCGGGCTTT TCCCTGCGCG GGGCGGGCGG CCGCAGCCCA GTAGCCCTGG CTTTGCTCCA GACCCGGCCC AGAGTGACAT CACCAAACTC CGCCGATCGG CGGGAGGGGG CCCTGAAATC CCCTAAAAAC AAAGTGAGTA ATCGGCGGAC CCACCCTCCA GGCGGGGCCG GGACCCGGGA GCTAGCCGAG CCACCGGGCA CGCCAGCCTC TTCGTCCTCG GCGAGGCGCG CACTTGGCCC CGACCCCCGG CAGCGGCTGT GCGTGCAGCC TCTTCGTCCT CCGCGCGGCG TGCACTTGCT CCCGGCCCCT GCGCCGGGCG GCGGCGGGGC AGCGCGCAGG CGCGGCCGGA TTCCGGGCAG TGACGCGACG GCGGGCCGCG CGGCGCATTT CCGCCTCTGG CGAATGGCTC GTCTGTAGTG CACGCCGCGG GCCCAGCTGC GACCCCGGCC CCGCCCCCGG GACCCCGGCC GCTTTCCGCC CCTTCCTGCG CCGACGGGCG CTGCGCAGGG AGCGGTCGGC CTGCCGGATG GTGGCGCGGG GCGGGGCAGG CGGGGAGGGG TCTGACTCAG TTTCCCCTCT GGGTGGAGGG GGTGGCCCTT GACTCAGCAT CCTCCCTGGG TGGGGGAGCA GAGGGAACCT TGACTCAGTT TCCCTCCACA CCGTCCATGG GGGCAGCTCT GGCTTCGTTT CCCTATTAGG GGAGAACGCA TCTGATTCAG TTTCCTCTCT GGAGGGAGGT GGGGCGTGCC CTCCCTCAAT TTTCCTTAAG AAAGTACAAC AGGCCCTGAT TCAGCTTCCC TCTGGAGAAG AAGGATTCCT TACCCCGTTT TCCCTCAAGA AGCAGGTGTT TCTGGTTCCT TTCCTCCACC CCTTAGTTTC ACCGCAGGTT CTAGGGAGCA GGTCCTGACT CAGTTCCCCT GGGAGGGAGC CTGGCTTCTC CTCTCCGTGG AGAGCAGATT CCTCCTTGTG AGAATGGGCA TTGGGTGTGT GCTGACCCTG ATCTCCCTCC CCTTTCAGAA CTGTTCCCCC TCATCTTCCC GGCAGGTAAG TGGCCCCCGC GGCGGTCGGG

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001145138, NM_001243984, NM_001243985, NM_021975
UniProt ID Q04206
Synonyms NFKB3; p65
Summary NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2011
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Documents
Resources
Other Versions
SKU Description Size Price
GA104066 RELA CRISPRa kit - CRISPR gene activation of human RELA proto-oncogene, NF-kB subunit 1 kit
$1,657.00
KN220780 RELA - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN220780BN RELA - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN220780LP RELA - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN420780 RELA - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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