Neurofibromin (NF1) Human Gene Knockout Kit (CRISPR)

SKU KN220378LP

NF1 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Vector: Luciferase-Puro
• Target Symbol: Neurofibromin
• Locus ID: 4763
• Components:

  KN220378G1, Neurofibromin gRNA vector 1 in pCas-Guide CRISPR vector

  KN220378G2, Neurofibromin gRNA vector 2 in pCas-Guide CRISPR vector

  KN220378LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  CTCCCGGGTC AGCTCTGGCA CTCGCCAGCT GAGCCCAGCG CCAGTCTAGG TGAGCCCCAC GGCGGTGAGG GACGCTCGCC AGACGGCCCA GAGGAGTTAG ATGACGTCAC CTCCAGGAGG ACTCGCTTTT TCATTAATGA AACCGGCCGG CGCGGGCGCA TGCGCGGCAG GCCGCCTTCC CTCTCGCTTC CCCCTCCCCT TTCCCAGCCG CGCTCTCAAT CTCTAGCTCG CTCGCGCTCC CTCTCCCCGG GCCGTGGAAA GGATCCCACT TCCGGTGGGG TGTCATGGCG GCGTCTCGGA CTGTGATGGC TGTGGGGAGA CGGCGCTAGT GGGGAGAGCG ACCAAGAGGC CCCCTCCCCT CCCCGGGTCC CCTTCCCCTA TCCCCCTCCC CCCAGCCTCC TTGCCAACGC CCCCTTTCCC TCTCCCCCTC CCGCTCGGCG CTGACCCCCC ATCCCCACCC CCGTGGGAAC ACTGGGAGCC TGCACTCCAC AGACCCTCTC CTTGCCTCTT CCCTCACCTC AGCCTCCGCT CCCCGCCCTC TTCCCGGCCC AGGGCGCCGG CCCACCCTTC CCTCCGCCGC CCCCCGGCCG CGGGGAGGAC AGTAGGTGAG GGGAGGTAGG AGCGGCCGCC TCCCCCGCGG CTGCCTCAGG CTCTGGAGGA AAGGAAGGGA AGGGAAGAGA AGGGAAGGGG GGATAAGTGG GGGTGGCCAA GGCGGGAGGT GAGGGGTAGG AGGGGACAGC TGGGAGCTTG GGCACCCTTT CCCTCCTAAG TCGGGGGGTG GGGCCTTGTC CCTGACCAGC CTCCGACCCT CCATCCCCTT TATCCCAGCC CTTCCGCTTG GAAATGGGGA TGAGTGACCT GGGGGCGCCT TTAGGGGCGC GCCATCTGGA TTTAATAATT ACCCCCTTAC TTTTGTTTTT GAGCAGAGGT GGGTAACGTT TAGGGGTTCT CTCTTCCTCT TCCCTCCGTG ACCCTCCTAA CTGGGGACGA GCGAACCCTA AGGCCTGTCT TATATACCCT CAATAGGAGA ACGATAACAT TCCGCCCCTC CCCCAGTTTC TTCAGCTTCC TCTCCTGGAG GGGGAAGAGC AGAAGAAAGC AAGGAAGGCA CAGGTTTTCT TACTTTCTGC TCCATCGTCT CATCCTGCCC CGAGAGCTTC AAGTGCACAC TCACTGCACA TCTTTGTGAT GCCCTACAAC

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000267, NM_001042492, NM_001128147
• UniProt ID: P21359
• Synonyms: NFNS; VRNF; WSS
• Summary: This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]
• Shipping: Ambient