Neurofibromin (NF1) Human Gene Knockout Kit (CRISPR)

SKU KN220378

NF1 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Vector: GFP-puro
• Target Symbol: Neurofibromin
• Locus ID: 4763
• Components:

  KN220378G1, Neurofibromin gRNA vector 1 in pCas-Guide CRISPR vector

  KN220378G2, Neurofibromin gRNA vector 2 in pCas-Guide CRISPR vector

  KN220378D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000267, NM_001042492, NM_001128147
• UniProt ID: P21359
• Synonyms: NFNS; VRNF; WSS
• Summary: This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]
• Shipping: Ambient