Filaggrin (FLG) Human Gene Knockout Kit (CRISPR)

CAT#: KN219792

FLG - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Filaggrin
• Locus ID: 2312
• Components:

  KN219792G1, Filaggrin gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAATTTCGGCAAATCCTGA

  KN219792G2, Filaggrin gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAAAGAGCTGAAGGAACTTC

  KN219792D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CCAGTCAGAA AAACCTTTCT CTTTAAATAT TGAAGAGCTA TTAAATCCAT CCTATACAAT GAAATATTAT AAACTTCATG AAATGTTTAA AAAACAAATA AAAAATTGAA TGAACTTTCA TTGAGTATCT TGTCATATGG CTAACTGGCT TTCAGAGAAT TGTGTCTTAG CAAATTAATC ATTTATTAAG TTAGCTTTTG GGGAAGTAAT TTTCAGTAAA TTTGTATAAG TAAATTTTAC TGCTTAAAAC CAGTCAGCCT TTCTAAATTC TTGTTGCTAG CAATGGCAAG ATCAGCAAGA GGGATGATCT TACCTAGAGC ACACTGAGGT CTGTGAGACT ACTAAGTCCA GCTGTAAGTG GGCACAAGGC CAAAAAATGG GCAATTTTAT TTAAGTCAGG AAAACTCTAT TCATTTGTTT TATGTAGATG GTAGAGAAAA GTTTCTCATG TCCATCTTCC ATTCTTAAAG ATGGATTACT TAGAGGAAAA TGTAGGTTTA AAATGTGGAA AATGATTGTA ATAATATAAC CTAGAAAAAA AGATGTATAA AAAAGTGCAT TTTCATTTCT TCTTTCTAGG TTCACATTTA TTGCCAAAAG AGGTAAGAGT CTCTGACAAG ACCAAAGATA GAGCATTTGT TTTTCTCATC AATAATCTAA GCAAGGGTTA TTTTGAGCTC TTTGTGAACT ATTTCTCTTA AAATAGGAAA AGAGTTAATC TTGTCTCAAA AGAGATAGAT TTTATTTTCT TCCCCTATAC CTGGATGTAT ATTATAAACA TAAAGACACC CTCAGAGAAA AAAATATAAA TATTTAAAAA TTAATGTAAA TTAGTTCTTA GTATTTTGGT ATTTATAATA TTCTCCCAAA CATCAACATT ACTAAGGGGA TTTTTGTTAA AGACAACTAA GTAATGAATT ATATACACTT CTGACTTTTA AAATAAGAAA AAGAGTCACT TACCCCATCA AATCTTGATA CCTTACTAGT CTACATCTTT GTTCACATAT AACACCTGAA TGAGGATGAG ACAAAAAAGT CTTAAAAAAA AAAAAGATTT TGTCCCACTT TTTAATACTC AAAGATCAGG TTCCTTAAAT TAACTTTGAA TTAATTGGCT GATAATGTGA TTCTGTCTGA TGCAGTCTCC CTCTGTGACT TCCCTCTGTA CAGAATCCAG ATGACCCAGA TATGGTTGAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_002016
• UniProt ID: P20930
• Synonyms: ATOD2
• Summary: The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]